6m0e
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Beijerinckia indica beta-fructosyltransferase complexed with fructose== | ==Beijerinckia indica beta-fructosyltransferase complexed with fructose== | ||
| - | <StructureSection load='6m0e' size='340' side='right'caption='[[6m0e]]' scene=''> | + | <StructureSection load='6m0e' size='340' side='right'caption='[[6m0e]], [[Resolution|resolution]] 1.35Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M0E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M0E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m0e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Beii9 Beii9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M0E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M0E FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m0e OCA], [http://pdbe.org/6m0e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m0e RCSB], [http://www.ebi.ac.uk/pdbsum/6m0e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m0e ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Bind_2021 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=395963 BEII9])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m0e OCA], [http://pdbe.org/6m0e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m0e RCSB], [http://www.ebi.ac.uk/pdbsum/6m0e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m0e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | An enzyme belonging to glycoside hydrolase family 68 (GH68) from Beijerinckia indica subsp. indica NBRC 3744 was expressed in Escherichia coli. Biochemical characterization showed that the enzyme was identified to be a beta-fructosyltransferase (BiBftA). Crystallization of a full-length BiBftA was initially attempted, but no crystals were obtained. We constructed a variant in which 5 residues (Pro199-Gly203) and 13 residues (Leu522-Gln534) in potentially flexible regions were deleted, and we successfully crystallized this variant BiBftA. BiBftA is composed of a five-bladed beta-propeller fold as in other GH68 enzymes. The structure of BiBftA in complex with fructose unexpectedly indicated that one beta-fructofuranose (beta-Fruf) molecule and one beta-fructopyranose molecule bind to the catalytic pocket. The orientation of beta-Fruf at subsite -1 is tilted from the orientation observed in most GH68 enzymes, presenting a second structure of a GH68 enzyme in complex with the tilted binding mode of beta-Fruf. | ||
| + | |||
| + | Crystal structure of a glycoside hydrolase family 68 beta-fructosyltransferase from Beijerinckia indica subsp. indica in complex with fructose.,Tonozuka T, Kitamura J, Nagaya M, Kawai R, Nishikawa A, Hirano K, Tamura K, Fujii T, Tochio T Biosci Biotechnol Biochem. 2020 Aug 4:1-13. doi: 10.1080/09168451.2020.1804317. PMID:32752982<ref>PMID:32752982</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6m0e" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Beii9]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Tonozuka T]] | + | [[Category: Levansucrase]] |
| + | [[Category: Tonozuka, T]] | ||
| + | [[Category: Fructooligosaccharide]] | ||
| + | [[Category: Gh68]] | ||
| + | [[Category: Hydrolase]] | ||
Revision as of 06:30, 19 August 2020
Beijerinckia indica beta-fructosyltransferase complexed with fructose
| |||||||||||
