6s8x
From Proteopedia
(Difference between revisions)
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==Crystal structure of the Rab-binding domain of FIP2== | ==Crystal structure of the Rab-binding domain of FIP2== | ||
| - | <StructureSection load='6s8x' size='340' side='right'caption='[[6s8x]]' scene=''> | + | <StructureSection load='6s8x' size='340' side='right'caption='[[6s8x]], [[Resolution|resolution]] 2.29Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S8X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S8X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6s8x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S8X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S8X FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s8x OCA], [http://pdbe.org/6s8x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s8x RCSB], [http://www.ebi.ac.uk/pdbsum/6s8x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s8x ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RAB11FIP2, KIAA0941 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s8x OCA], [http://pdbe.org/6s8x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s8x RCSB], [http://www.ebi.ac.uk/pdbsum/6s8x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s8x ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/RFIP2_HUMAN RFIP2_HUMAN]] A Rab11 effector protein acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Binds preferentially to phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA). Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity.<ref>PMID:12364336</ref> <ref>PMID:15304524</ref> <ref>PMID:16775013</ref> <ref>PMID:19542231</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The small GTPases Rab11, Rab14 and Rab25 regulate membrane trafficking through the recruitment of Rab11 family-interacting proteins (FIPs) to endocytic compartments. FIPs are multi-domain effector proteins that have a highly conserved Rab-binding domain (RBD) at their C-termini. Several structures of complexes of Rab11 with RBDs have previously been determined, including those of Rab11-FIP2 and Rab11-FIP3. In addition, the structures of the Rab14-FIP1 and Rab25-FIP2 complexes have been determined. All of the RBD structures contain a central parallel coiled coil in the RBD that binds to the switch 1 and switch 2 regions of the Rab. Here, the crystal structure of the uncomplexed RBD of FIP2 is presented at 2.3 A resolution. The structure reveals antiparallel alpha-helices that associate through polar interactions. These include a remarkable stack of arginine residues within a four-helix bundle in the crystal lattice. | ||
| + | |||
| + | Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2.,Kearney AM, Khan AR Acta Crystallogr F Struct Biol Commun. 2020 Aug 1;76(Pt 8):357-363. doi:, 10.1107/S2053230X20009164. Epub 2020 Jul 28. PMID:32744247<ref>PMID:32744247</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6s8x" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Kearney | + | [[Category: Kearney, A M]] |
| - | [[Category: Khan | + | [[Category: Khan, A R]] |
| + | [[Category: Effector protein]] | ||
| + | [[Category: Endosomal trafficking]] | ||
| + | [[Category: Membrane trafficking]] | ||
| + | [[Category: Rab small gtpase]] | ||
| + | [[Category: Rab-binding domain]] | ||
| + | [[Category: Signaling protein]] | ||
Current revision
Crystal structure of the Rab-binding domain of FIP2
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