6yj5
From Proteopedia
(Difference between revisions)
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==Focused refinement cryo-EM structure of the yeast mitochondrial complex I sub-stoichiometric sulfur transferase subunit== | ==Focused refinement cryo-EM structure of the yeast mitochondrial complex I sub-stoichiometric sulfur transferase subunit== | ||
| - | <StructureSection load='6yj5' size='340' side='right'caption='[[6yj5]]' scene=''> | + | <StructureSection load='6yj5' size='340' side='right'caption='[[6yj5]], [[Resolution|resolution]] 3.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YJ5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yj5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18942 Atcc 18942]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YJ5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yj5 OCA], [http://pdbe.org/6yj5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yj5 RCSB], [http://www.ebi.ac.uk/pdbsum/6yj5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yj5 ProSAT]</span></td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6yj4|6yj4]]</td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">B0I71DRAFT_128700, YALI1_F30877g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4952 ATCC 18942])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yj5 OCA], [http://pdbe.org/6yj5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yj5 RCSB], [http://www.ebi.ac.uk/pdbsum/6yj5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yj5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mitochondrial complex I powers ATP synthesis by oxidative phosphorylation, exploiting the energy from ubiquinone reduction by NADH to drive protons across the energy-transducing inner membrane. Recent cryo-EM analyses of mammalian and yeast complex I have revolutionized structural and mechanistic knowledge and defined structures in different functional states. Here, we describe a 2.7-A-resolution structure of the 42-subunit complex I from the yeast Yarrowia lipolytica containing 275 structured water molecules. We identify a proton-relay pathway for ubiquinone reduction and water molecules that connect mechanistically crucial elements and constitute proton-translocation pathways through the membrane. By comparison with known structures, we deconvolute structural changes governing the mammalian 'deactive transition' (relevant to ischemia-reperfusion injury) and their effects on the ubiquinone-binding site and a connected cavity in ND1. Our structure thus provides important insights into catalysis by this enigmatic respiratory machine. | ||
| + | |||
| + | Mitochondrial complex I structure reveals ordered water molecules for catalysis and proton translocation.,Grba DN, Hirst J Nat Struct Mol Biol. 2020 Aug 3. pii: 10.1038/s41594-020-0473-x. doi:, 10.1038/s41594-020-0473-x. PMID:32747785<ref>PMID:32747785</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6yj5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Atcc 18942]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Grba D]] | + | [[Category: Grba, D]] |
| - | [[Category: Hirst J]] | + | [[Category: Hirst, J]] |
| + | [[Category: Complex i]] | ||
| + | [[Category: Nadh:ubiquinone oxidoreductase]] | ||
| + | [[Category: Sub-stoichiometric]] | ||
| + | [[Category: Sulfur transferase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 06:42, 19 August 2020
Focused refinement cryo-EM structure of the yeast mitochondrial complex I sub-stoichiometric sulfur transferase subunit
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