7c2l
From Proteopedia
(Difference between revisions)
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==S protein of SARS-CoV-2 in complex bound with 4A8== | ==S protein of SARS-CoV-2 in complex bound with 4A8== | ||
| - | <StructureSection load='7c2l' size='340' side='right'caption='[[7c2l]]' scene=''> | + | <StructureSection load='7c2l' size='340' side='right'caption='[[7c2l]], [[Resolution|resolution]] 3.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C2L OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C2L FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7c2l]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/2019-ncov 2019-ncov] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C2L OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C2L FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c2l OCA], [http://pdbe.org/7c2l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c2l RCSB], [http://www.ebi.ac.uk/pdbsum/7c2l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c2l ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">S, 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2697049 2019-nCoV])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c2l OCA], [http://pdbe.org/7c2l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c2l RCSB], [http://www.ebi.ac.uk/pdbsum/7c2l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c2l ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Developing therapeutics against SARS-CoV-2 could be guided by the distribution of epitopes, not only on the receptor binding domain (RBD) of the Spike (S) protein, but also across the full Spike (S) protein. We isolated and characterized monoclonal antibodies (mAbs) from ten convalescent COVID-19 patients. Three mAbs showed neutralizing activities against authentic SARS-CoV-2. An mAb, named 4A8, exhibits high neutralization potency against both authentic and pseudotyped SARS-CoV-2, but does not bind the RBD. We defined the epitope of 4A8 as the N terminal domain (NTD) of the S protein by determining its cryo-EM structure in complex with the S protein to an overall resolution of 3.1 Angstrom and local resolution of 3.3 Angstrom for the 4A8-NTD interface. This points to the NTD as a promising target for therapeutic mAbs against COVID-19. | ||
| + | |||
| + | A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2.,Chi X, Yan R, Zhang J, Zhang G, Zhang Y, Hao M, Zhang Z, Fan P, Dong Y, Yang Y, Chen Z, Guo Y, Zhang J, Li Y, Song X, Chen Y, Xia L, Fu L, Hou L, Xu J, Yu C, Li J, Zhou Q, Chen W Science. 2020 Jun 22. pii: science.abc6952. doi: 10.1126/science.abc6952. PMID:32571838<ref>PMID:32571838</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7c2l" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: 2019-ncov]] | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Guo | + | [[Category: Guo, Y Y]] |
| - | [[Category: Li | + | [[Category: Li, Y N]] |
| - | [[Category: Xia L]] | + | [[Category: Xia, L]] |
| - | [[Category: Yan | + | [[Category: Yan, R H]] |
| - | [[Category: Zhang | + | [[Category: Zhang, Y Y]] |
| - | [[Category: Zhou Q]] | + | [[Category: Zhou, Q]] |
| + | [[Category: Ace2-b0at1 complex]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Membrane protein-immune system complex]] | ||
Revision as of 06:43, 19 August 2020
S protein of SARS-CoV-2 in complex bound with 4A8
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