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5me3

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Revision as of 07:16, 19 August 2020

Structure of the Scc2 C-terminus

Structural highlights

5me3 is a 6 chain structure with sequence from Ashgo. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	SCC2, AGL133W (ASHGO)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SCC2_ASHGO] Plays a structural role in chromatin. Probably involved in sister chromatid cohesion, possibly by interacting with the cohesin complex (By similarity).

Publication Abstract from PubMed

The functions of cohesin are central to genome integrity, chromosome organization and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2-Scc4 complex; however, little is known about how it stimulates the cohesion-loading activity. Here we determine the large 'hook' structure of Scc2 responsible for catalysing cohesin loading. We identify key Scc2 surfaces that are crucial for cohesin loading in vivo. With the aid of previously determined structures and homology modelling, we derive a pseudo-atomic structure of the full-length Scc2-Scc4 complex. Finally, using recombinantly purified Scc2-Scc4 and cohesin, we performed crosslinking mass spectrometry and interaction assays that suggest Scc2-Scc4 uses its modular structure to make multiple contacts with cohesin.

Structure of the cohesin loader Scc2.,Chao WC, Murayama Y, Munoz S, Jones AW, Wade BO, Purkiss AG, Hu XW, Borg A, Snijders AP, Uhlmann F, Singleton MR Nat Commun. 2017 Jan 6;8:13952. doi: 10.1038/ncomms13952. PMID:28059076^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chao WC, Murayama Y, Munoz S, Jones AW, Wade BO, Purkiss AG, Hu XW, Borg A, Snijders AP, Uhlmann F, Singleton MR. Structure of the cohesin loader Scc2. Nat Commun. 2017 Jan 6;8:13952. doi: 10.1038/ncomms13952. PMID:28059076 doi:http://dx.doi.org/10.1038/ncomms13952

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5me3"

@@ Line 1: / Line 1: @@
 ==Structure of the Scc2 C-terminus==
-<StructureSection load='5me3' size='340' side='right' caption='[[5me3]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
+<StructureSection load='5me3' size='340' side='right'caption='[[5me3]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5me3]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ME3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ME3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5me3]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ashgo Ashgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ME3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ME3 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5me3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5me3 OCA], [http://pdbe.org/5me3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5me3 RCSB], [http://www.ebi.ac.uk/pdbsum/5me3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5me3 ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCC2, AGL133W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284811 ASHGO])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5me3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5me3 OCA], [http://pdbe.org/5me3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5me3 RCSB], [http://www.ebi.ac.uk/pdbsum/5me3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5me3 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Ashgo]]
+[[Category: Large Structures]]
 [[Category: Chao, W C.H]]
 [[Category: Singleton, M R]]

5me3

From Proteopedia

Revision as of 07:16, 19 August 2020

Structure of the Scc2 C-terminus

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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