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5mg8

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Revision as of 07:18, 19 August 2020

Crystal structure of the S.pombe Smc5/6 hinge domain

Structural highlights

5mg8 is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SMC5_SCHPO] Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. Plays a critical role in meiosis. [SMC6_SCHPO] Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. Plays a critical role in meiosis.^[1]

Publication Abstract from PubMed

The Structural Maintenance of Chromosomes (SMC) complexes: cohesin, condensin and Smc5/6 are involved in the organization of higher-order chromosome structure-which is essential for accurate chromosome duplication and segregation. Each complex is scaffolded by a specific SMC protein dimer (heterodimer in eukaryotes) held together via their hinge domains. Here we show that the Smc5/6-hinge, like those of cohesin and condensin, also forms a toroidal structure but with distinctive subunit interfaces absent from the other SMC complexes; an unusual 'molecular latch' and a functional 'hub'. Defined mutations in these interfaces cause severe phenotypic effects with sensitivity to DNA-damaging agents in fission yeast and reduced viability in human cells. We show that the Smc5/6-hinge complex binds preferentially to ssDNA and that this interaction is affected by both 'latch' and 'hub' mutations, suggesting a key role for these unique features in controlling DNA association by the Smc5/6 complex.

Specialized interfaces of Smc5/6 control hinge stability and DNA association.,Alt A, Dang HQ, Wells OS, Polo LM, Smith MA, McGregor GA, Welte T, Lehmann AR, Pearl LH, Murray JM, Oliver AW Nat Commun. 2017 Jan 30;8:14011. doi: 10.1038/ncomms14011. PMID:28134253^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Verkade HM, Bugg SJ, Lindsay HD, Carr AM, O'Connell MJ. Rad18 is required for DNA repair and checkpoint responses in fission yeast. Mol Biol Cell. 1999 Sep;10(9):2905-18. PMID:10473635
↑ Alt A, Dang HQ, Wells OS, Polo LM, Smith MA, McGregor GA, Welte T, Lehmann AR, Pearl LH, Murray JM, Oliver AW. Specialized interfaces of Smc5/6 control hinge stability and DNA association. Nat Commun. 2017 Jan 30;8:14011. doi: 10.1038/ncomms14011. PMID:28134253 doi:http://dx.doi.org/10.1038/ncomms14011

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5mg8"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the S.pombe Smc5/6 hinge domain==
-<StructureSection load='5mg8' size='340' side='right' caption='[[5mg8]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
+<StructureSection load='5mg8' size='340' side='right'caption='[[5mg8]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5mg8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MG8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MG8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5mg8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MG8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MG8 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mg8 OCA], [http://pdbe.org/5mg8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mg8 RCSB], [http://www.ebi.ac.uk/pdbsum/5mg8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mg8 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mg8 OCA], [http://pdbe.org/5mg8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mg8 RCSB], [http://www.ebi.ac.uk/pdbsum/5mg8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mg8 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Alt, A]]
 [[Category: Oliver, A W]]

5mg8

From Proteopedia

Revision as of 07:18, 19 August 2020

Crystal structure of the S.pombe Smc5/6 hinge domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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