5n77

From Proteopedia

(Difference between revisions)

Revision as of 11:58, 26 August 2020

Crystal structure of the cytosolic domain of the CorA magnesium channel from Escherichia coli in complex with magnesium

Structural highlights

5n77 is a 5 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CORA_ECOLI] Mediates influx of magnesium ions. Can also mediate cobalt and manganese uptake (PubMed:780341). Alternates between open and closed states. Activated by low cytoplasmic Mg(2+) levels. Inactive when cytoplasmic Mg(2+) levels are high (By similarity).[UniProtKB:Q9WZ31]^[1]

Publication Abstract from PubMed

Structures of the Mg2+ bound (closed) and apo (open) states of CorA suggests that channel gating is accomplished by rigid-body motions between symmetric and asymmetric assemblies of the cytosolic portions of the five subunits in response to ligand (Mg2+) binding/unbinding at interfacial sites. Here, we structurally and biochemically characterize the isolated cytosolic domain from Escherichia coli CorA. The data reveal an Mg2+-ligand binding site located in a novel position between each of the five subunits and two Mg2+ ions trapped inside the pore. Soaking experiments show that cobalt hexammine outcompetes Mg2+ at the pore site closest to the membrane. This represents the first structural information of how an analog of hexa-hydrated Mg2+ (and competitive inhibitor of CorA) associates to the CorA pore. Biochemical data on the isolated cytoplasmic domain and full-length protein suggests that gating of the CorA channel is governed cooperatively.

Structure and Cooperativity of the Cytosolic Domain of the CorA Mg2+ Channel from Escherichia coli.,Lerche M, Sandhu H, Flockner L, Hogbom M, Rapp M Structure. 2017 Aug 1;25(8):1175-1186.e4. doi: 10.1016/j.str.2017.05.024. Epub, 2017 Jun 29. PMID:28669631^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Park MH, Wong BB, Lusk JE. Mutants in three genes affecting transport of magnesium in Escherichia coli: genetics and physiology. J Bacteriol. 1976 Jun;126(3):1096-103. PMID:780341
↑ Lerche M, Sandhu H, Flockner L, Hogbom M, Rapp M. Structure and Cooperativity of the Cytosolic Domain of the CorA Mg2+ Channel from Escherichia coli. Structure. 2017 Aug 1;25(8):1175-1186.e4. doi: 10.1016/j.str.2017.05.024. Epub, 2017 Jun 29. PMID:28669631 doi:http://dx.doi.org/10.1016/j.str.2017.05.024

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5n77"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the cytosolic domain of the CorA magnesium channel from Escherichia coli in complex with magnesium==
-<StructureSection load='5n77' size='340' side='right' caption='[[5n77]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='5n77' size='340' side='right'caption='[[5n77]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5n77]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N77 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5n77]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N77 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N77 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n77 OCA], [http://pdbe.org/5n77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n77 RCSB], [http://www.ebi.ac.uk/pdbsum/5n77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n77 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n77 OCA], [http://pdbe.org/5n77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n77 RCSB], [http://www.ebi.ac.uk/pdbsum/5n77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n77 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Flockner, L]]
 [[Category: Hogbom, M]]

5n77

From Proteopedia

Revision as of 11:58, 26 August 2020

Crystal structure of the cytosolic domain of the CorA magnesium channel from Escherichia coli in complex with magnesium

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox