5n7j

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(Difference between revisions)

Revision as of 11:58, 26 August 2020

Crystal structure of Neisseria polysaccharea amylosucrase mutant efficient for the synthesis of controlled size maltooligosaccharides

Structural highlights

5n7j is a 1 chain structure with sequence from "neisseria_polysacchareae"_riou_et_al._1983 "neisseria polysacchareae" riou et al. 1983. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Gene:	ams ("Neisseria polysacchareae" Riou et al. 1983)
Activity:	Amylosucrase, with EC number 2.4.1.4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.^[1]

Publication Abstract from PubMed

Amylosucrase from Neisseria polysaccharea naturally catalyzes the synthesis of alpha-1,4 glucans from sucrose. The product profile is quite polydisperse, ranging from soluble chains called maltooligosaccharides to high-molecular weight insoluble amylose. This enzyme was recently subjected to engineering of its active site to enable recognition of non-natural acceptor substrates. Libraries of variants were constructed and screened on sucrose, allowing the identification of a mutant that showed a 6-fold enhanced activity toward sucrose compared to the wild-type enzyme. Furthermore, its product profile was unprecedented, as only soluble maltooligosaccharides of controlled size chains (2<DP<21) with a narrow polydispersity were observed. This variant, containing 9 mutations in the active site, was characterized at both biochemical and structural levels. Its x-ray structure was determined and further investigated by molecular dynamics to understand the molecular origins of its higher activity on sucrose and higher production of small maltooligosaccharides, with a totally abolished insoluble glucan synthesis.

Engineering of anp efficient mutant of Neisseria polysaccharea amylosucrase for the synthesis of controlled size maltooligosaccharides.,Verges A, Barbe S, Cambon E, Moulis C, Tranier S, Remaud-Simeon M, Andre I Carbohydr Polym. 2017 Oct 1;173:403-411. doi: 10.1016/j.carbpol.2017.06.011. Epub, 2017 Jun 7. PMID:28732882^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ De Montalk GP, Remaud-Simeon M, Willemot RM, Planchot V, Monsan P. Sequence analysis of the gene encoding amylosucrase from Neisseria polysaccharea and characterization of the recombinant enzyme. J Bacteriol. 1999 Jan;181(2):375-81. PMID:9882648
↑ Verges A, Barbe S, Cambon E, Moulis C, Tranier S, Remaud-Simeon M, Andre I. Engineering of anp efficient mutant of Neisseria polysaccharea amylosucrase for the synthesis of controlled size maltooligosaccharides. Carbohydr Polym. 2017 Oct 1;173:403-411. doi: 10.1016/j.carbpol.2017.06.011. Epub, 2017 Jun 7. PMID:28732882 doi:http://dx.doi.org/10.1016/j.carbpol.2017.06.011

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5n7j"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Neisseria polysaccharea amylosucrase mutant efficient for the synthesis of controlled size maltooligosaccharides==
-<StructureSection load='5n7j' size='340' side='right' caption='[[5n7j]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='5n7j' size='340' side='right'caption='[[5n7j]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5n7j]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N7J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5n7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"neisseria_polysacchareae"_riou_et_al._1983 "neisseria polysacchareae" riou et al. 1983]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N7J FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MLR:MALTOTRIOSE'>MLR</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ams ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489 "Neisseria polysacchareae" Riou et al. 1983])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n7j OCA], [http://pdbe.org/5n7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n7j RCSB], [http://www.ebi.ac.uk/pdbsum/5n7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n7j ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n7j OCA], [http://pdbe.org/5n7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n7j RCSB], [http://www.ebi.ac.uk/pdbsum/5n7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n7j ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Neisseria polysacchareae riou et al. 1983]]
 [[Category: Amylosucrase]]
+[[Category: Large Structures]]
 [[Category: Tranier, S]]
 [[Category: Verges, A]]

5n7j

From Proteopedia

Revision as of 11:58, 26 August 2020

Crystal structure of Neisseria polysaccharea amylosucrase mutant efficient for the synthesis of controlled size maltooligosaccharides

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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