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5o1z

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Revision as of 12:16, 26 August 2020

Structure of Nrd1 RNA binding domain in complex with RNA (CGUAAA)

Structural highlights

5o1z is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NRD1_YEAST] Plays a role in sequence-specific regulation of nuclear pre-mRNA abundance.

Publication Abstract from PubMed

Transcription termination of non-coding RNAs is regulated in yeast by a complex of three RNA binding proteins: Nrd1, Nab3 and Sen1. Nrd1 is central in this process by interacting with Rbp1 of RNA polymerase II, Trf4 of TRAMP and GUAA/G terminator sequences. We lack structural data for the last of these binding events. We determined the structures of Nrd1 RNA binding domain and its complexes with three GUAA-containing RNAs, characterized RNA binding energetics and tested rationally designed mutants in vivo. The Nrd1 structure shows an RRM domain fused with a second alpha/beta domain that we name split domain (SD), because it is formed by two non-consecutive segments at each side of the RRM. The GUAA interacts with both domains and with a pocket of water molecules, trapped between the two stacking adenines and the SD. Comprehensive binding studies demonstrate for the first time that Nrd1 has a slight preference for GUAA over GUAG and genetic and functional studies suggest that Nrd1 RNA binding domain might play further roles in non-coding RNAs transcription termination.

The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition.,Franco-Echevarria E, Gonzalez-Polo N, Zorrilla S, Martinez-Lumbreras S, Santiveri CM, Campos-Olivas R, Sanchez M, Calvo O, Gonzalez B, Perez-Canadillas JM Nucleic Acids Res. 2017 Sep 29;45(17):10293-10305. doi: 10.1093/nar/gkx685. PMID:28973465^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Franco-Echevarria E, Gonzalez-Polo N, Zorrilla S, Martinez-Lumbreras S, Santiveri CM, Campos-Olivas R, Sanchez M, Calvo O, Gonzalez B, Perez-Canadillas JM. The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition. Nucleic Acids Res. 2017 Sep 29;45(17):10293-10305. doi: 10.1093/nar/gkx685. PMID:28973465 doi:http://dx.doi.org/10.1093/nar/gkx685

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5o1z"

@@ Line 1: / Line 1: @@
 ==Structure of Nrd1 RNA binding domain in complex with RNA (CGUAAA)==
-<StructureSection load='5o1z' size='340' side='right' caption='[[5o1z]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
+<StructureSection load='5o1z' size='340' side='right'caption='[[5o1z]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5o1z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O1Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O1Z FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5o1z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O1Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5O1Z FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o1z OCA], [http://pdbe.org/5o1z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o1z RCSB], [http://www.ebi.ac.uk/pdbsum/5o1z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o1z ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5o1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o1z OCA], [http://pdbe.org/5o1z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o1z RCSB], [http://www.ebi.ac.uk/pdbsum/5o1z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o1z ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Franco-Echevarria, E]]
 [[Category: Gonzalez, B]]

5o1z

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Revision as of 12:16, 26 August 2020

Structure of Nrd1 RNA binding domain in complex with RNA (CGUAAA)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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