1chr

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[[Image:1chr.jpg|left|200px]]
[[Image:1chr.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1chr |SIZE=350|CAPTION= <scene name='initialview01'>1chr</scene>, resolution 3.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1chr", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chloromuconate_cycloisomerase Chloromuconate cycloisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.7 5.5.1.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1chr| PDB=1chr | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1chr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1chr OCA], [http://www.ebi.ac.uk/pdbsum/1chr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1chr RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF CHLOROMUCONATE CYCLOISOMERASE FROM ALCALIGENES EUTROPHUS JMP134 (PJP4) AT 3 ANGSTROMS RESOLUTION'''
'''CRYSTAL STRUCTURE OF CHLOROMUCONATE CYCLOISOMERASE FROM ALCALIGENES EUTROPHUS JMP134 (PJP4) AT 3 ANGSTROMS RESOLUTION'''
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[[Category: Schloemann, M.]]
[[Category: Schloemann, M.]]
[[Category: Stezowski, J J.]]
[[Category: Stezowski, J J.]]
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[[Category: isomerase]]
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[[Category: Isomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:44:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:21:56 2008''
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Revision as of 09:44, 2 May 2008

Image:1chr.jpg

Template:STRUCTURE 1chr

CRYSTAL STRUCTURE OF CHLOROMUCONATE CYCLOISOMERASE FROM ALCALIGENES EUTROPHUS JMP134 (PJP4) AT 3 ANGSTROMS RESOLUTION


Overview

Chloromuconate cycloisomerase (E.C. 5.5.1.7) is an enzyme involved in the 2,4-dichlorophenoxyacetate degradation pathway of Alcaligenes eutrophus JMP134 (pJP4). The crystal structure of this protein was determined at 3 A resolution by molecular-replacement techniques using atomic coordinates from the reported crystal structure of the homologous muconate cycloisomerase (E.C. 5.5.1.1) from Pseudomonas putida as the search model (42% identical positions in the sequences). Structure refinement by simulated-annealing and restrained least-squares techniques converged at R = 0.195. In the crystals studied, space group I4, the protein is present as two octamers per unit cell with two subunits per asymmetric unit. Each subunit consists of two globular domains, one of which forms an alpha/beta-barrel. Comparison of this structure with that of muconate cycloisomerase reveals the reasons for the altered substrate specificity of chloromuconate cycloisomerase. Marked differences are observed in polarity, accessibility and hydrogen-bonding potential in the channel leading into the active site as well as in the active center itself.

About this Structure

1CHR is a Single protein structure of sequence from Cupriavidus necator. Full crystallographic information is available from OCA.

Reference

Crystal structure of chloromuconate cycloisomerase from Alcaligenes eutrophus JMP134 (pJP4) at 3 A resolution., Hoier H, Schlomann M, Hammer A, Glusker JP, Carrell HL, Goldman A, Stezowski JJ, Heinemann U, Acta Crystallogr D Biol Crystallogr. 1994 Jan 1;50(Pt 1):75-84. PMID:15299479 Page seeded by OCA on Fri May 2 12:44:44 2008

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