5oxv

From Proteopedia

(Difference between revisions)

Revision as of 07:33, 2 September 2020

Structure of the 4_601_157 tetranucleosome (C2 form)

Structural highlights

5oxv is a 18 chain structure with sequence from [1] and African clawed frog. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5oy7
Gene:	hist1h2aj, LOC494591 (African clawed frog)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[H4_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [H2B11_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [H32_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Publication Abstract from PubMed

Chromatin fiber organization is implicated in processes such as transcription, DNA repair and chromosome segregation, but how nucleosomes interact to form higher order structure remains poorly understood. We solved two crystal structures of tetranucleosomes with approximately 11 base pair DNA linker length at 5.8 and 6.7A resolution. Minimal intramolecular nucleosome-nucleosome interactions result in a fiber model resembling a flat ribbon that is compatible with a two-start helical architecture, and that exposes histone and DNA surfaces to the environment. The differences in the two structures combined with electron microscopy reveal heterogeneous structural states, and we used site-specific chemical crosslinking to assess the diversity of nucleosome-nucleosome interactions through identification of structure-sensitive crosslink sites that provide a means to characterize fibers in solution. The chromatin fiber architectures observed here provide a basis for understanding heterogeneous chromatin higher order structures as they occur in a genomic context.

Capturing structural heterogeneity in chromatin fibers.,Ekundayo B, Richmond TJ, Schalch T J Mol Biol. 2017 Sep 9. pii: S0022-2836(17)30424-2. doi:, 10.1016/j.jmb.2017.09.002. PMID:28893533^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ekundayo B, Richmond TJ, Schalch T. Capturing structural heterogeneity in chromatin fibers. J Mol Biol. 2017 Sep 9. pii: S0022-2836(17)30424-2. doi:, 10.1016/j.jmb.2017.09.002. PMID:28893533 doi:http://dx.doi.org/10.1016/j.jmb.2017.09.002

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5oxv"

@@ Line 1: / Line 1: @@
 ==Structure of the 4_601_157 tetranucleosome (C2 form)==
-<StructureSection load='5oxv' size='340' side='right' caption='[[5oxv]], [[Resolution|resolution]] 6.72&Aring;' scene=''>
+<StructureSection load='5oxv' size='340' side='right'caption='[[5oxv]], [[Resolution|resolution]] 6.72&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5oxv]] is a 18 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OXV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5oxv]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OXV FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5oy7|5oy7]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxv OCA], [http://pdbe.org/5oxv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oxv RCSB], [http://www.ebi.ac.uk/pdbsum/5oxv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxv ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hist1h2aj, LOC494591 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5oxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxv OCA], [http://pdbe.org/5oxv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oxv RCSB], [http://www.ebi.ac.uk/pdbsum/5oxv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5oxv" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Histone 3D structures|Histone 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: African clawed frog]]
+[[Category: Large Structures]]
 [[Category: Ekundayo, B]]
 [[Category: Schalch, T]]

5oxv

From Proteopedia

Revision as of 07:33, 2 September 2020

Structure of the 4_601_157 tetranucleosome (C2 form)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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