1pjy

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Revision as of 12:17, 8 November 2007

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Solution structure of the HIV-1 frameshift inducing stem-loop RNA

Overview

The translation of reverse transcriptase and other essential viral, proteins from the HIV-1 Pol mRNA requires a programmed -1 ribosomal, frameshift. This frameshift is induced by two highly conserved elements, within the HIV-1 mRNA: a slippery sequence comprised of a UUUUUUA, heptamer, and a downstream stem-loop structure. We have determined the, structure of the HIV-1 frameshift inducing RNA stem-loop, using, multidimensional heteronuclear nuclear magnetic resonance (NMR) methods., The 22 nucleotide RNA solution structure [root mean squared deviation, (r.m.s.d.) = 1.2 A] was determined from 475 nuclear Overhauser effect, (NOE)-derived distance restrains, 20 residual dipolar couplings and direct, detection of hydrogen bonds via scalar couplings. We find that the, frameshift inducing stem-loop is an A-form helix capped by a structured, ACAA tetraloop. The ACAA tetraloop is stabilized by an equilateral 5' and, 3' stacking pattern, a sheared A-A pair and a cross-strand hydrogen bond., Unexpectedly, the ACAA tetraloop structure is nearly identical to a known, tetraloop fold, previously identified in the RNase III recognition site, from Saccharomyces cerevisiae.

About this Structure

1PJY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:12888491

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