This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6w90
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==De novo designed NTF2 fold protein NT-9== | ==De novo designed NTF2 fold protein NT-9== | ||
| - | <StructureSection load='6w90' size='340' side='right'caption='[[6w90]]' scene=''> | + | <StructureSection load='6w90' size='340' side='right'caption='[[6w90]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W90 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W90 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6w90]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W90 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W90 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w90 OCA], [http://pdbe.org/6w90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w90 RCSB], [http://www.ebi.ac.uk/pdbsum/6w90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w90 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w90 OCA], [http://pdbe.org/6w90 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w90 RCSB], [http://www.ebi.ac.uk/pdbsum/6w90 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w90 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Naturally occurring proteins vary the precise geometries of structural elements to create distinct shapes optimal for function. We present a computational design method, loop-helix-loop unit combinatorial sampling (LUCS), that mimics nature's ability to create families of proteins with the same overall fold but precisely tunable geometries. Through near-exhaustive sampling of loop-helix-loop elements, LUCS generates highly diverse geometries encompassing those found in nature but also surpassing known structure space. Biophysical characterization showed that 17 (38%) of 45 tested LUCS designs encompassing two different structural topologies were well folded, including 16 with designed non-native geometries. Four experimentally solved structures closely matched the designs. LUCS greatly expands the designable structure space and offers a new paradigm for designing proteins with tunable geometries that may be customizable for novel functions. | ||
| + | |||
| + | Expanding the space of protein geometries by computational design of de novo fold families.,Pan X, Thompson MC, Zhang Y, Liu L, Fraser JS, Kelly MJS, Kortemme T Science. 2020 Aug 28;369(6507):1132-1136. doi: 10.1126/science.abc0881. PMID:32855341<ref>PMID:32855341</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6w90" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Fraser | + | [[Category: Synthetic construct sequences]] |
| - | [[Category: Kortemme T]] | + | [[Category: Fraser, J S]] |
| - | [[Category: Liu L]] | + | [[Category: Kortemme, T]] |
| - | [[Category: Pan X]] | + | [[Category: Liu, L]] |
| - | [[Category: Thompson | + | [[Category: Pan, X]] |
| + | [[Category: Thompson, M C]] | ||
| + | [[Category: De novo fold family]] | ||
| + | [[Category: De novo protein]] | ||
| + | [[Category: Geometric sampling]] | ||
| + | [[Category: Loop-helix-loop motif]] | ||
| + | [[Category: Protein design]] | ||
Revision as of 09:55, 9 September 2020
De novo designed NTF2 fold protein NT-9
| |||||||||||
