6jc8
From Proteopedia
(Difference between revisions)
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<StructureSection load='6jc8' size='340' side='right'caption='[[6jc8]], [[Resolution|resolution]] 2.25Å' scene=''> | <StructureSection load='6jc8' size='340' side='right'caption='[[6jc8]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6jc8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JC8 OCA]. For a <b>guided tour on the structure components</b> use [http:// | + | <table><tr><td colspan='2'>[[6jc8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Actinoalloteichus_sp._wh1-2216-6 Actinoalloteichus sp. wh1-2216-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JC8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JC8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PL6:(E)-N-({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYLIDENE)-L-GLUTAMIC+ACID'>PL6</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PL6:(E)-N-({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYLIDENE)-L-GLUTAMIC+ACID'>PL6</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jc8 OCA], [http://pdbe.org/6jc8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jc8 RCSB], [http://www.ebi.ac.uk/pdbsum/6jc8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jc8 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Amine compounds biosynthesis using omega-transaminases has received considerable attention in the pharmaceutical industry. However, the application of omega-transaminases was hampered by the fundamental challenge of severe by-product inhibition. Here, we report that omega-transaminase CrmG from Actinoalloteichus cyanogriseus WH1-2216-6 is insensitive to inhibition from by-product alpha-ketoglutarate or pyruvate. Combined with structural and QM/MM studies, we establish the detailed catalytic mechanism for CrmG. Our structural and biochemical studies reveal that the roof of the active site in PMP-bound CrmG is flexible, which will facilitate the PMP or by-product to dissociate from PMP-bound CrmG. Our results also show that amino acceptor caerulomycin M (CRM M), but not alpha-ketoglutarate or pyruvate, can form strong interactions with the roof of the active site in PMP-bound CrmG. Based on our results, we propose that the flexible roof of the active site in PMP-bound CrmG may facilitate CrmG to overcome inhibition from the by-product. | ||
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| + | Structural studies reveal flexible roof of active site responsible for omega-transaminase CrmG overcoming by-product inhibition.,Xu J, Tang X, Zhu Y, Yu Z, Su K, Zhang Y, Dong Y, Zhu W, Zhang C, Wu R, Liu J Commun Biol. 2020 Aug 19;3(1):455. doi: 10.1038/s42003-020-01184-w. PMID:32814814<ref>PMID:32814814</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6jc8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Actinoalloteichus sp. wh1-2216-6]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Liu, J]] | [[Category: Liu, J]] | ||
Revision as of 10:01, 16 September 2020
Crystal structure of aminotransferase CrmG from Actinoalloteichus sp. WH1-2216-6 in complex with amino donor L-Glu
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