6lnp
From Proteopedia
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==Crystal structure of citrate Biosensor== | ==Crystal structure of citrate Biosensor== | ||
| - | <StructureSection load='6lnp' size='340' side='right'caption='[[6lnp]]' scene=''> | + | <StructureSection load='6lnp' size='340' side='right'caption='[[6lnp]], [[Resolution|resolution]] 2.99Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LNP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LNP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lnp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LNP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LNP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lnp OCA], [http://pdbe.org/6lnp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lnp RCSB], [http://www.ebi.ac.uk/pdbsum/6lnp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lnp ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">citA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lnp OCA], [http://pdbe.org/6lnp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lnp RCSB], [http://www.ebi.ac.uk/pdbsum/6lnp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lnp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Motivated by the growing recognition of citrate as a central metabolite in a variety of biological processes associated with healthy and diseased cellular states, we have developed a series of high-performance genetically encoded citrate biosensors suitable for imaging of citrate concentrations in mammalian cells. The design of these biosensors was guided by structural studies of the citrate-responsive sensor histidine kinase and took advantage of the same conformational changes proposed to propagate from the binding domain to the catalytic domain. Following extensive engineering based on a combination of structure guided mutagenesis and directed evolution, we produced an inverse-response biosensor (DeltaF/F min approximately 18) designated Citroff1 and a direct-response biosensor (DeltaF/F min approximately 9) designated Citron1. We report the X-ray crystal structure of Citron1 and demonstrate the utility of both biosensors for qualitative and quantitative imaging of steady-state and pharmacologically perturbed citrate concentrations in live cells. | ||
| + | |||
| + | High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors for Citrate.,Zhao Y, Shen Y, Wen Y, Campbell RE ACS Cent Sci. 2020 Aug 26;6(8):1441-1450. doi: 10.1021/acscentsci.0c00518. Epub, 2020 Jul 9. PMID:32875085<ref>PMID:32875085</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6lnp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Histidine kinase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Campbell R]] | + | [[Category: Campbell, R]] |
| - | [[Category: Wen Y]] | + | [[Category: Wen, Y]] |
| + | [[Category: Biosensor]] | ||
| + | [[Category: Citrate]] | ||
| + | [[Category: Fluorescent protein]] | ||
| + | [[Category: Genetically encoded]] | ||
Revision as of 10:05, 16 September 2020
Crystal structure of citrate Biosensor
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