6zgi
From Proteopedia
(Difference between revisions)
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==Furin Cleaved Spike Protein of SARS-CoV-2 in Closed Conformation== | ==Furin Cleaved Spike Protein of SARS-CoV-2 in Closed Conformation== | ||
| - | <StructureSection load='6zgi' size='340' side='right'caption='[[6zgi]]' scene=''> | + | <StructureSection load='6zgi' size='340' side='right'caption='[[6zgi]], [[Resolution|resolution]] 2.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZGI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZGI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zgi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/2019-ncov 2019-ncov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZGI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZGI FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zgi OCA], [http://pdbe.org/6zgi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zgi RCSB], [http://www.ebi.ac.uk/pdbsum/6zgi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zgi ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">S, 2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2697049 2019-nCoV])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zgi OCA], [http://pdbe.org/6zgi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zgi RCSB], [http://www.ebi.ac.uk/pdbsum/6zgi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zgi ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2]] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | SARS-CoV-2 is thought to have emerged from bats, possibly via a secondary host. Here, we investigate the relationship of spike (S) glycoprotein from SARS-CoV-2 with the S protein of a closely related bat virus, RaTG13. We determined cryo-EM structures for RaTG13 S and for both furin-cleaved and uncleaved SARS-CoV-2 S; we compared these with recently reported structures for uncleaved SARS-CoV-2 S. We also biochemically characterized their relative stabilities and affinities for the SARS-CoV-2 receptor ACE2. Although the overall structures of human and bat virus S proteins are similar, there are key differences in their properties, including a more stable precleavage form of human S and about 1,000-fold tighter binding of SARS-CoV-2 to human receptor. These observations suggest that cleavage at the furin-cleavage site decreases the overall stability of SARS-CoV-2 S and facilitates the adoption of the open conformation that is required for S to bind to the ACE2 receptor. | ||
| + | |||
| + | SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects.,Wrobel AG, Benton DJ, Xu P, Roustan C, Martin SR, Rosenthal PB, Skehel JJ, Gamblin SJ Nat Struct Mol Biol. 2020 Jul 9. pii: 10.1038/s41594-020-0468-7. doi:, 10.1038/s41594-020-0468-7. PMID:32647346<ref>PMID:32647346</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zgi" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[SARS-CoV-2 protein | + | *[[SARS-CoV-2 spike protein fusion transformation|SARS-CoV-2 spike protein fusion transformation]] |
| + | *[[SARS-CoV-2 spike protein priming by furin|SARS-CoV-2 spike protein priming by furin]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: 2019-ncov]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Benton | + | [[Category: Benton, D J]] |
| - | [[Category: Gamblin | + | [[Category: Gamblin, S J]] |
| - | [[Category: Rosenthal | + | [[Category: Rosenthal, P B]] |
| - | [[Category: Wrobel | + | [[Category: Wrobel, A G]] |
| + | [[Category: Coronavirus]] | ||
| + | [[Category: Sars-cov-2]] | ||
| + | [[Category: Spike]] | ||
| + | [[Category: Viral protein]] | ||
| + | [[Category: Virus glycoprotein]] | ||
Current revision
Furin Cleaved Spike Protein of SARS-CoV-2 in Closed Conformation
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