1haa

From Proteopedia

(Difference between revisions)

Revision as of 10:34, 16 September 2020

A beta-Hairpin Structure in a 13-mer Peptide that Binds a-Bungarotoxin with High Affinity and Neutralizes its Toxicity

Structural highlights

1haa is a 2 chain structure with sequence from Bungarus multicinctus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1abt, 1bxp, 1hn7, 1hoy, 2btx, 1haj
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NXL1_BUNMU] Neurotoxin. Binds and inhibits nicotinic acetylcholine receptors. Compared to alpha-neurotoxins, kappa-neurotoxins bind more strongly to neuronal receptors, and less strongly to muscle receptors.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Snake-venom alpha-bungarotoxin is a member of the alpha-neurotoxin family that binds with very high affinity to the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction. The structure of the complex between alpha-bungarotoxin and a 13-mer peptide (WRYYESSLEPYPD) that binds the toxin with high affinity, thus inhibiting its interactions with AChR with an IC(50) of 2 nM, has been solved by (1)H-NMR spectroscopy. The bound peptide folds into a beta-hairpin structure created by two antiparallel beta-strands, which combine with the already existing triple-stranded beta-sheet of the toxin to form a five-stranded intermolecular, antiparallel beta-sheet. Peptide residues Y3(P), E5(P), and L8(P) have the highest intermolecular contact area, indicating their importance in the binding of alpha-bungarotoxin; W1(P), R2(P), and Y4(P) also contribute significantly to the binding. A large number of characteristic hydrogen bonds and electrostatic and hydrophobic interactions are observed in the complex. The high-affinity peptide exhibits inhibitory potency that is better than any known peptide derived from AChR, and is equal to that of the whole alpha-subunit of AChR. The high degree of sequence similarity between the peptide and various types of AChRs implies that the binding mode found within the complex might possibly mimic the receptor binding to the toxin. The design of the high-affinity peptide was based on our previous findings: (i) the detection of a lead peptide (MRYYESSLKSYPD) that binds alpha-bungarotoxin, using a phage-display peptide library, (ii) the information about the three-dimensional structure of alpha-bungarotoxin/lead-peptide complex, and (iii) the amino acid sequence analysis of different AChRs.

A beta -hairpin structure in a 13-mer peptide that binds alpha -bungarotoxin with high affinity and neutralizes its toxicity.,Scherf T, Kasher R, Balass M, Fridkin M, Fuchs S, Katchalski-Katzir E Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6629-34. Epub 2001 May 29. PMID:11381118^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Grant GA, Chiappinelli VA. kappa-Bungarotoxin: complete amino acid sequence of a neuronal nicotinic receptor probe. Biochemistry. 1985 Mar 12;24(6):1532-7. PMID:3986193
↑ Scherf T, Kasher R, Balass M, Fridkin M, Fuchs S, Katchalski-Katzir E. A beta -hairpin structure in a 13-mer peptide that binds alpha -bungarotoxin with high affinity and neutralizes its toxicity. Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6629-34. Epub 2001 May 29. PMID:11381118 doi:10.1073/pnas.111164298

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1haa"

@@ Line 1: / Line 1: @@
 ==A beta-Hairpin Structure in a 13-mer Peptide that Binds a-Bungarotoxin with High Affinity and Neutralizes its Toxicity==
-<StructureSection load='1haa' size='340' side='right' caption='[[1haa]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+<StructureSection load='1haa' size='340' side='right'caption='[[1haa]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1haa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HAA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1haa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HAA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1HAA FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1abt|1abt]], [[1bxp|1bxp]], [[1hn7|1hn7]], [[1hoy|1hoy]], [[2btx|2btx]], [[1haj|1haj]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1haa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1haa OCA], [http://pdbe.org/1haa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1haa RCSB], [http://www.ebi.ac.uk/pdbsum/1haa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1haa ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1haa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1haa OCA], [http://pdbe.org/1haa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1haa RCSB], [http://www.ebi.ac.uk/pdbsum/1haa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1haa ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Bungarotoxin|Bungarotoxin]]
+*[[Bungarotoxin 3D structures|Bungarotoxin 3D structures]]
 == References ==
 <references/>
@@ Line 36: / Line 36: @@
 </StructureSection>
 [[Category: Bungarus multicinctus]]
+[[Category: Large Structures]]
 [[Category: Balass, M]]
 [[Category: Fridkin, M]]

1haa

From Proteopedia

Revision as of 10:34, 16 September 2020

A beta-Hairpin Structure in a 13-mer Peptide that Binds a-Bungarotoxin with High Affinity and Neutralizes its Toxicity

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox