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5t56

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Revision as of 11:22, 16 September 2020

[3]catenane from MccJ25 G12R/I13C/G21C lasso peptide

Structural highlights

5t56 is a 4 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MCJA_ECOLX] Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). May inhibit transcription by binding in RNAP secondary channel and blocking nucleotide substrates access to the catalytic center. Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption. Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Lasso peptides exist naturally in a threaded state as [1]rotaxanes, and we reasoned that lasso peptides cleaved in their loop region could serve as building blocks for catenanes. Mutagenesis of the lasso peptide microcin J25 (MccJ25) with two cysteine residues followed by cleavage of the peptide with trypsin led to a [2]rotaxane structure that self-assembled into a [3]catenane and [4]catenanes at room temperature in aqueous solution. The [3]catenane represents the smallest ring size of a catenane composed solely of polypeptide segments. The NMR structure of the [3]catenane was determined, suggesting that burial of hydrophobic residues may be a driving force for assembly of the catenane structure.

Self-Assembly of Catenanes from Lasso Peptides.,Allen CD, Link AJ J Am Chem Soc. 2016 Nov 2;138(43):14214-14217. Epub 2016 Oct 21. PMID:27768305^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rintoul MR, de Arcuri BF, Salomon RA, Farias RN, Morero RD. The antibacterial action of microcin J25: evidence for disruption of cytoplasmic membrane energization in Salmonella newport. FEMS Microbiol Lett. 2001 Nov 13;204(2):265-70. PMID:11731133
↑ Delgado MA, Rintoul MR, Farias RN, Salomon RA. Escherichia coli RNA polymerase is the target of the cyclopeptide antibiotic microcin J25. J Bacteriol. 2001 Aug;183(15):4543-50. PMID:11443089 doi:http://dx.doi.org/10.1128/JB.183.15.4543-4550.2001
↑ Yuzenkova J, Delgado M, Nechaev S, Savalia D, Epshtein V, Artsimovitch I, Mooney RA, Landick R, Farias RN, Salomon R, Severinov K. Mutations of bacterial RNA polymerase leading to resistance to microcin j25. J Biol Chem. 2002 Dec 27;277(52):50867-75. Epub 2002 Oct 24. PMID:12401787 doi:http://dx.doi.org/10.1074/jbc.M209425200
↑ Allen CD, Link AJ. Self-Assembly of Catenanes from Lasso Peptides. J Am Chem Soc. 2016 Nov 2;138(43):14214-14217. Epub 2016 Oct 21. PMID:27768305 doi:http://dx.doi.org/10.1021/jacs.6b09454

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5t56"

@@ Line 1: / Line 1: @@
 ==[3]catenane from MccJ25 G12R/I13C/G21C lasso peptide==
-<StructureSection load='5t56' size='340' side='right' caption='[[5t56]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='5t56' size='340' side='right'caption='[[5t56]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5t56]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T56 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T56 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5t56]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T56 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5T56 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t56 OCA], [http://pdbe.org/5t56 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t56 RCSB], [http://www.ebi.ac.uk/pdbsum/5t56 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t56 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5t56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t56 OCA], [http://pdbe.org/5t56 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t56 RCSB], [http://www.ebi.ac.uk/pdbsum/5t56 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t56 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Allen, C D]]
 [[Category: Link, A J]]

5t56

From Proteopedia

Revision as of 11:22, 16 September 2020

[3]catenane from MccJ25 G12R/I13C/G21C lasso peptide

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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