6m4p
From Proteopedia
(Difference between revisions)
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==Cytochrome P450 monooxygenase StvP2 substrate-bound structure== | ==Cytochrome P450 monooxygenase StvP2 substrate-bound structure== | ||
| - | <StructureSection load='6m4p' size='340' side='right'caption='[[6m4p]]' scene=''> | + | <StructureSection load='6m4p' size='340' side='right'caption='[[6m4p]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M4P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M4P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m4p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27465 Atcc 27465]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M4P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M4P FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m4p OCA], [http://pdbe.org/6m4p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m4p RCSB], [http://www.ebi.ac.uk/pdbsum/6m4p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m4p ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F4O:6-methoxy-streptovaricin+C'>F4O</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">stvP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68270 ATCC 27465])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m4p OCA], [http://pdbe.org/6m4p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m4p RCSB], [http://www.ebi.ac.uk/pdbsum/6m4p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m4p ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Streptovaricin C is a naphthalenic ansamycin antibiotic structurally similar to rifamycins with potential anti-MRSA bioactivities. However, the formation mechanism of the most fascinating and bioactivity-related methylenedioxy bridge (MDB) moiety in streptovaricins is unclear. Based on genetic and biochemical evidences, we herein clarify that the P450 enzyme StvP2 catalyzes the MDB formation in streptovaricins, with an atypical substrate inhibition kinetics. Furthermore, X-ray crystal structures in complex with substrate and structure-based mutagenesis reveal the intrinsic details of the enzymatic reaction. The mechanism of MDB formation is proposed to be an intramolecular nucleophilic substitution resulting from the hydroxylation by the heme core and the keto-enol tautomerization via a crucial catalytic triad (Asp89-His92-Arg72) in StvP2. In addition, in vitro reconstitution uncovers that C6-O-methylation and C4-O-acetylation of streptovaricins are necessary prerequisites for the MDB formation. This work provides insight for the MDB formation and adds evidence in support of the functional versatility of P450 enzymes. | ||
| + | |||
| + | Uncovering the cytochrome P450-catalyzed methylenedioxy bridge formation in streptovaricins biosynthesis.,Sun G, Hu C, Mei Q, Luo M, Chen X, Li Z, Liu Y, Deng Z, Zhang Z, Sun Y Nat Commun. 2020 Sep 9;11(1):4501. doi: 10.1038/s41467-020-18336-5. PMID:32908132<ref>PMID:32908132</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6m4p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Atcc 27465]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Chen X]] | + | [[Category: Chen, X]] |
| - | [[Category: Deng Z]] | + | [[Category: Deng, Z]] |
| - | [[Category: Hu C]] | + | [[Category: Hu, C]] |
| - | [[Category: Li Z]] | + | [[Category: Li, Z]] |
| - | [[Category: Liu Y]] | + | [[Category: Liu, Y]] |
| - | [[Category: Luo M]] | + | [[Category: Luo, M]] |
| - | [[Category: Mei Q]] | + | [[Category: Mei, Q]] |
| - | [[Category: Sun G]] | + | [[Category: Sun, G]] |
| - | [[Category: Sun Y]] | + | [[Category: Sun, Y]] |
| - | [[Category: Zhang Z]] | + | [[Category: Zhang, Z]] |
| + | [[Category: Antibiotic]] | ||
| + | [[Category: P450 anti-mrsa streptovaricin c methylenedioxy bridge stvp2 substrate-bound structure]] | ||
Revision as of 11:23, 23 September 2020
Cytochrome P450 monooxygenase StvP2 substrate-bound structure
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