6x8n
From Proteopedia
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==Crystal Structure of H49A ABLE mutant== | ==Crystal Structure of H49A ABLE mutant== | ||
| - | <StructureSection load='6x8n' size='340' side='right'caption='[[6x8n]]' scene=''> | + | <StructureSection load='6x8n' size='340' side='right'caption='[[6x8n]], [[Resolution|resolution]] 1.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X8N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X8N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6x8n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X8N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X8N FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x8n OCA], [http://pdbe.org/6x8n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x8n RCSB], [http://www.ebi.ac.uk/pdbsum/6x8n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x8n ProSAT]</span></td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6w70|6w70]], [[6w6x|6w6x]]</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x8n OCA], [http://pdbe.org/6x8n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x8n RCSB], [http://www.ebi.ac.uk/pdbsum/6x8n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x8n ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The de novo design of proteins that bind highly functionalized small molecules represents a great challenge. To enable computational design of binders, we developed a unit of protein structure-a van der Mer (vdM)-that maps the backbone of each amino acid to statistically preferred positions of interacting chemical groups. Using vdMs, we designed six de novo proteins to bind the drug apixaban; two bound with low and submicromolar affinity. X-ray crystallography and mutagenesis confirmed a structure with a precisely designed cavity that forms favorable interactions in the drug-protein complex. vdMs may enable design of functional proteins for applications in sensing, medicine, and catalysis. | ||
| + | |||
| + | A defined structural unit enables de novo design of small-molecule-binding proteins.,Polizzi NF, DeGrado WF Science. 2020 Sep 4;369(6508):1227-1233. doi: 10.1126/science.abb8330. PMID:32883865<ref>PMID:32883865</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6x8n" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Polizzi | + | [[Category: Synthetic construct sequences]] |
| + | [[Category: Polizzi, N F]] | ||
| + | [[Category: 4-helix bundle]] | ||
| + | [[Category: De novo]] | ||
| + | [[Category: De novo protein]] | ||
| + | [[Category: Ligand-binding]] | ||
Revision as of 11:30, 23 September 2020
Crystal Structure of H49A ABLE mutant
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