6y5u
From Proteopedia
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==Rv1045 nucleotidyltransferase toxin from Mycobacterium tuberculosis== | ==Rv1045 nucleotidyltransferase toxin from Mycobacterium tuberculosis== | ||
| - | <StructureSection load='6y5u' size='340' side='right'caption='[[6y5u]]' scene=''> | + | <StructureSection load='6y5u' size='340' side='right'caption='[[6y5u]], [[Resolution|resolution]] 1.59Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y5U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y5U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6y5u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y5U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Y5U FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y5u OCA], [http://pdbe.org/6y5u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y5u RCSB], [http://www.ebi.ac.uk/pdbsum/6y5u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y5u ProSAT]</span></td></tr> | + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv1045 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6y5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y5u OCA], [http://pdbe.org/6y5u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y5u RCSB], [http://www.ebi.ac.uk/pdbsum/6y5u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y5u ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Toxin-antitoxin systems are widespread stress-responsive elements, many of whose functions remain largely unknown. Here, we characterize the four DUF1814-family nucleotidyltransferase-like toxins (MenT1-4) encoded by the human pathogen Mycobacterium tuberculosis. Toxin MenT3 inhibited growth of M. tuberculosis when not antagonized by its cognate antitoxin, MenA3. We solved the structures of toxins MenT3 and MenT4 to 1.6 and 1.2 A resolution, respectively, and identified the biochemical activity and target of MenT3. MenT3 blocked in vitro protein expression and prevented tRNA charging in vivo. MenT3 added pyrimidines (C or U) to the 3'-CCA acceptor stems of uncharged tRNAs and exhibited strong substrate specificity in vitro, preferentially targeting tRNA(Ser) from among the 45 M. tuberculosis tRNAs. Our study identifies a previously unknown mechanism that expands the range of enzymatic activities used by bacterial toxins, uncovering a new way to block protein synthesis and potentially treat tuberculosis and other infections. | ||
| + | |||
| + | A nucleotidyltransferase toxin inhibits growth of Mycobacterium tuberculosis through inactivation of tRNA acceptor stems.,Cai Y, Usher B, Gutierrez C, Tolcan A, Mansour M, Fineran PC, Condon C, Neyrolles O, Genevaux P, Blower TR Sci Adv. 2020 Jul 29;6(31):eabb6651. doi: 10.1126/sciadv.abb6651. eCollection, 2020 Jul. PMID:32923609<ref>PMID:32923609</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6y5u" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Blower | + | [[Category: Myctu]] |
| - | [[Category: Usher B]] | + | [[Category: Blower, T R]] |
| + | [[Category: Usher, B]] | ||
| + | [[Category: Nucleotidyltransferase toxin-antitoxin system mycobacterium tuberculosis]] | ||
| + | [[Category: Toxin]] | ||
Revision as of 11:32, 23 September 2020
Rv1045 nucleotidyltransferase toxin from Mycobacterium tuberculosis
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