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5u6n

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Revision as of 12:28, 23 September 2020

Crystal structure of UDP-glucosyltransferase, UGT74F2 (T15S), with UDP and salicylic acid

Structural highlights

5u6n is a 2 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	5u6s, 5u6m
Gene:	UGT74F2, GT, SAGT1, SGT1, At2g43820, F18O19.7 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[U74F2_ARATH] Glycosyltransferase that glucosylates benzoic acid and derivatives. Substrate preference is benzoic acid > salicylic acid (SA) > 3-hydroxybenzoic acid > 4-hydroxybenzoic acid. Catalyzes the formation of both SA 2-O-beta-D-glucoside (SAG) and SA glucose ester (SGE). Has high affinity for the tryptophan precursor anthranilate. Catalyzes the formation of anthranilate glucose ester. Is the major source of this activity in the plant.^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

Salicylic acid (SA) is a signaling molecule utilized by plants in response to various stresses. Through conjugation with small organic molecules such as glucose, an inactive form of SA is generated which can be transported into and stored in plant vacuoles. In the model organism Arabidopsis thaliana, SA glucose conjugates are formed by two homologous enzymes (UGT74F1 and UGT74F2) that transfer glucose from UDP-glucose to SA. Despite being 77% identical and with conserved active site residues, these enzymes catalyze the formation of different products: UGT74F1 forms salicylic acid glucoside (SAG), while UGT74F2 forms primarily salicylic acid glucose ester (SGE). The position of the glucose on the aglycone determines how SA is stored, further metabolized, and contributes to a defense response. We determined the crystal structures of the UGT74F2 wild-type and T15S mutant enzymes, in different substrate/product complexes. On the basis of the crystal structures and the effect on enzyme activity of mutations in the SA binding site, we propose the catalytic mechanism of SGE and SAG formation and that SA binds to the active site in two conformations, with each enzyme selecting a certain binding mode of SA. Additionally, we show that two threonines are key determinants of product specificity.

Differences in salicylic acid glucose conjugations by UGT74F1 and UGT74F2 from Arabidopsis thaliana.,George Thompson AM, Iancu CV, Neet KE, Dean JV, Choe JY Sci Rep. 2017 Apr 20;7:46629. doi: 10.1038/srep46629. PMID:28425481^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lim EK, Doucet CJ, Li Y, Elias L, Worrall D, Spencer SP, Ross J, Bowles DJ. The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates. J Biol Chem. 2002 Jan 4;277(1):586-92. Epub 2001 Oct 18. PMID:11641410 doi:http://dx.doi.org/10.1074/jbc.M109287200
↑ Quiel JA, Bender J. Glucose conjugation of anthranilate by the Arabidopsis UGT74F2 glucosyltransferase is required for tryptophan mutant blue fluorescence. J Biol Chem. 2003 Feb 21;278(8):6275-81. Epub 2002 Dec 9. PMID:12475971 doi:http://dx.doi.org/10.1074/jbc.M211822200
↑ Song JT. Induction of a salicylic acid glucosyltransferase, AtSGT1, is an early disease response in Arabidopsis thaliana. Mol Cells. 2006 Oct 31;22(2):233-8. PMID:17085977
↑ Song JT, Koo YJ, Seo HS, Kim MC, Choi YD, Kim JH. Overexpression of AtSGT1, an Arabidopsis salicylic acid glucosyltransferase, leads to increased susceptibility to Pseudomonas syringae. Phytochemistry. 2008 Mar;69(5):1128-34. doi: 10.1016/j.phytochem.2007.12.010., Epub 2008 Jan 28. PMID:18226820 doi:http://dx.doi.org/10.1016/j.phytochem.2007.12.010
↑ Eudes A, Bozzo GG, Waller JC, Naponelli V, Lim EK, Bowles DJ, Gregory JF 3rd, Hanson AD. Metabolism of the folate precursor p-aminobenzoate in plants: glucose ester formation and vacuolar storage. J Biol Chem. 2008 May 30;283(22):15451-9. doi: 10.1074/jbc.M709591200. Epub 2008 , Apr 2. PMID:18385129 doi:http://dx.doi.org/10.1074/jbc.M709591200
↑ George Thompson AM, Iancu CV, Neet KE, Dean JV, Choe JY. Differences in salicylic acid glucose conjugations by UGT74F1 and UGT74F2 from Arabidopsis thaliana. Sci Rep. 2017 Apr 20;7:46629. doi: 10.1038/srep46629. PMID:28425481 doi:http://dx.doi.org/10.1038/srep46629

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5u6n"

@@ Line 1: / Line 1: @@
 ==Crystal structure of UDP-glucosyltransferase, UGT74F2 (T15S), with UDP and salicylic acid==
-<StructureSection load='5u6n' size='340' side='right' caption='[[5u6n]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='5u6n' size='340' side='right'caption='[[5u6n]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5u6n]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U6N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U6N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5u6n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U6N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5U6N FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=LB2:3-O-BETA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSE'>LB2</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5u6s|5u6s]], [[5u6m|5u6m]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u6n OCA], [http://pdbe.org/5u6n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u6n RCSB], [http://www.ebi.ac.uk/pdbsum/5u6n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u6n ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UGT74F2, GT, SAGT1, SGT1, At2g43820, F18O19.7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5u6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u6n OCA], [http://pdbe.org/5u6n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u6n RCSB], [http://www.ebi.ac.uk/pdbsum/5u6n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u6n ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 20: @@
 </div>
 <div class="pdbe-citations 5u6n" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Arath]]
+[[Category: Large Structures]]
 [[Category: Choe, J]]
 [[Category: Dean, J V]]

5u6n

From Proteopedia

Revision as of 12:28, 23 September 2020

Crystal structure of UDP-glucosyltransferase, UGT74F2 (T15S), with UDP and salicylic acid

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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