6jxk
From Proteopedia
(Difference between revisions)
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<StructureSection load='6jxk' size='340' side='right'caption='[[6jxk]], [[Resolution|resolution]] 4.30Å' scene=''> | <StructureSection load='6jxk' size='340' side='right'caption='[[6jxk]], [[Resolution|resolution]] 4.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6jxk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXK OCA]. For a <b>guided tour on the structure components</b> use [http:// | + | <table><tr><td colspan='2'>[[6jxk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JXK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MF4:TETRAFLUOROMAGNESATE(2-)'>MF4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MF4:TETRAFLUOROMAGNESATE(2-)'>MF4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ATP4A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG]), ATP4B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 PIG])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)/K(+)-exchanging_ATPase H(+)/K(+)-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.2.2.19 7.2.2.19] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)/K(+)-exchanging_ATPase H(+)/K(+)-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.2.2.19 7.2.2.19] </span></td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jxk OCA], [http://pdbe.org/6jxk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jxk RCSB], [http://www.ebi.ac.uk/pdbsum/6jxk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jxk ProSAT]</span></td></tr> |
</table> | </table> | ||
== Disease == | == Disease == | ||
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG]] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach. | [[http://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG]] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The gastric proton pump (H(+),K(+)-ATPase), a P-type ATPase responsible for gastric acidification, mediates electro-neutral exchange of H(+) and K(+) coupled with ATP hydrolysis, but with an as yet undetermined transport stoichiometry. Here we show crystal structures at a resolution of 2.5 A of the pump in the E2-P transition state, in which the counter-transporting cation is occluded. We found a single K(+) bound to the cation-binding site of the H(+),K(+)-ATPase, indicating an exchange of 1H(+)/1K(+) per hydrolysis of one ATP molecule. This fulfills the energy requirement for the generation of a six pH unit gradient across the membrane. The structural basis of K(+) recognition is resolved and supported by molecular dynamics simulations, establishing how the H(+),K(+)-ATPase overcomes the energetic challenge to generate an H(+) gradient of more than a million-fold-one of the highest cation gradients known in mammalian tissue-across the membrane. | ||
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| + | A single K(+)-binding site in the crystal structure of the gastric proton pump.,Yamamoto K, Dubey V, Irie K, Nakanishi H, Khandelia H, Fujiyoshi Y, Abe K Elife. 2019 Aug 22;8. pii: 47701. doi: 10.7554/eLife.47701. PMID:31436534<ref>PMID:31436534</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6jxk" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[ATPase 3D structures|ATPase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pig]] | ||
[[Category: Abe, K]] | [[Category: Abe, K]] | ||
[[Category: Irie, K]] | [[Category: Irie, K]] | ||
Revision as of 07:19, 30 September 2020
Rb+-bound E2-MgF state of the gastric proton pump (Wild-type)
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Categories: Large Structures | Pig | Abe, K | Irie, K | Yamamoto, K | Gastric | Ion pump | Membrane protein | P-type atpase | Proton pump
