6x4s
From Proteopedia
(Difference between revisions)
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==MCU-EMRE complex of a metazoan mitochondrial calcium uniporter== | ==MCU-EMRE complex of a metazoan mitochondrial calcium uniporter== | ||
| - | <StructureSection load='6x4s' size='340' side='right'caption='[[6x4s]]' scene=''> | + | <StructureSection load='6x4s' size='340' side='right'caption='[[6x4s]], [[Resolution|resolution]] 3.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X4S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X4S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6x4s]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Red_flour_beetle Red flour beetle]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X4S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6X4S FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x4s OCA], [http://pdbe.org/6x4s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x4s RCSB], [http://www.ebi.ac.uk/pdbsum/6x4s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x4s ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TcasGA2_TC012057 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7070 Red flour beetle])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x4s OCA], [http://pdbe.org/6x4s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x4s RCSB], [http://www.ebi.ac.uk/pdbsum/6x4s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x4s ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/D6WIX5_TRICA D6WIX5_TRICA]] Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes a pore-forming and calcium-conducting subunit. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.[RuleBase:RU367035] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The proteins MCU and EMRE form the minimal functional unit of the mitochondrial calcium uniporter complex in metazoans, a highly selective and tightly controlled Ca(2+) channel of the inner mitochondrial membrane that regulates cellular metabolism. Here we present functional reconstitution of an MCU-EMRE complex from the red flour beetle, Tribolium castaneum, and a cryo-EM structure of the complex at 3.5A resolution. Using a novel assay, we demonstrate robust Ca(2+) uptake into proteoliposomes containing the purified complex. Uptake is dependent on EMRE and also on the mitochondrial lipid cardiolipin. The structure reveals a tetrameric channel with a single ion pore. EMRE is located at the periphery of the transmembrane domain and associates primarily with the first transmembrane helix of MCU. Coiled coil and juxtamembrane domains within the matrix portion of the complex adopt markedly different conformations than in a structure of a human MCU-EMRE complex, suggesting that the structures represent different conformations of these functionally similar metazoan channels. | ||
| + | |||
| + | Structure and Reconstitution of a MCU-EMRE Mitochondrial Ca(2+) Uniporter Complex.,Wang C, Baradaran R, Long SB J Mol Biol. 2020 Aug 22. pii: S0022-2836(20)30507-6. doi:, 10.1016/j.jmb.2020.08.013. PMID:32841658<ref>PMID:32841658</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6x4s" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Baradaran R]] | + | [[Category: Red flour beetle]] |
| - | [[Category: Long | + | [[Category: Baradaran, R]] |
| - | [[Category: Wang C]] | + | [[Category: Long, S B]] |
| + | [[Category: Wang, C]] | ||
| + | [[Category: Calcium channel]] | ||
| + | [[Category: Emre]] | ||
| + | [[Category: Ion channel]] | ||
| + | [[Category: Mcu]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Mitochondria]] | ||
| + | [[Category: Mitochondrial calcium uniporter]] | ||
Revision as of 07:44, 30 September 2020
MCU-EMRE complex of a metazoan mitochondrial calcium uniporter
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