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6st8

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Revision as of 06:36, 7 October 2020

Crystal structure of the strawberry pathogenesis-related 10 (PR-10) Fra a 1.02 protein

Structural highlights

6st8 is a 2 chain structure with sequence from Fraan. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5amw
Gene:	FRAA2 (FRAAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FRA12_FRAAN] Involved in the control of flavonoid biosynthesis in fruits, probably by binding directly to natural flavonoids (By similarity). Binds the natural flavonoid myricetin with affinities in the low micromolar range (PubMed:24133217).[UniProtKB:D0E0C7]^[1]

Publication Abstract from PubMed

Although strawberries are highly appreciated fruits, their intake can induce allergic reactions in atopic patients. These reactions can be due to the patient s previous sensitization to the major birch-pollen allergen Bet v 1, by which, IgE generated in response to Bet v 1 crossreacts with the structurally-related strawberry Fra a 1 protein family. Fra a 1.02 is the most expressed paralog in ripe strawberries and is highly allergenic. To better understand the molecular mechanisms regulating this allergic response, we have determined the three-dimensional structure of Fra a 1.02 and four site-directed mutants that were designed based on their positions in potential epitopes. Fra a 1.02 and mutants conform to the START fold. We show that the cross-reactivity of all the mutant variants to IgE from patients allergic to Bet v 1 was significantly reduced without altering the conserved structural fold, so that they could potentially be used as hypoallergenic Fra a 1 variants for the generation of vaccines against strawberry allergy in atopic patients.

STRUCTURAL BASIS FOR THE ALLERGENICITY OF Fra a 1.02 IN STRAWBERRY FRUITS.,Orozco-Navarrete B, Kaczmarska Z, Dupeux F, Garrido-Aranda M, Pott D, Diaz-Perales A, Casanal A, Marquez JA, Valpuesta V, Merchante C J Agric Food Chem. 2019 Nov 27. doi: 10.1021/acs.jafc.9b05714. PMID:31774998^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Casanal A, Zander U, Munoz C, Dupeux F, Luque I, Botella MA, Schwab W, Valpuesta V, Marquez JA. The Strawberry Pathogenesis-Related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates. J Biol Chem. 2013 Oct 16. PMID:24133217 doi:http://dx.doi.org/10.1074/jbc.M113.501528
↑ Orozco-Navarrete B, Kaczmarska Z, Dupeux F, Garrido-Aranda M, Pott D, Diaz-Perales A, Casanal A, Marquez JA, Valpuesta V, Merchante C. STRUCTURAL BASIS FOR THE ALLERGENICITY OF Fra a 1.02 IN STRAWBERRY FRUITS. J Agric Food Chem. 2019 Nov 27. doi: 10.1021/acs.jafc.9b05714. PMID:31774998 doi:http://dx.doi.org/10.1021/acs.jafc.9b05714

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6st8"

@@ Line 3: / Line 3: @@
 <StructureSection load='6st8' size='340' side='right'caption='[[6st8]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6st8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ST8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ST8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6st8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fraan Fraan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ST8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ST8 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5amw|5amw]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6st8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6st8 OCA], [http://pdbe.org/6st8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6st8 RCSB], [http://www.ebi.ac.uk/pdbsum/6st8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6st8 ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FRAA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3747 FRAAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6st8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6st8 OCA], [http://pdbe.org/6st8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6st8 RCSB], [http://www.ebi.ac.uk/pdbsum/6st8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6st8 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Fraan]]
 [[Category: Large Structures]]
 [[Category: Casanal, A]]

6st8

From Proteopedia

Revision as of 06:36, 7 October 2020

Crystal structure of the strawberry pathogenesis-related 10 (PR-10) Fra a 1.02 protein

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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