6um9
From Proteopedia
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==Gypsy Moth Pheromone-binding protein 1 (LdisPBP1) NMR Structure at pH 4.5== | ==Gypsy Moth Pheromone-binding protein 1 (LdisPBP1) NMR Structure at pH 4.5== | ||
| - | <StructureSection load='6um9' size='340' side='right'caption='[[6um9]]' scene=''> | + | <StructureSection load='6um9' size='340' side='right'caption='[[6um9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[6um9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gypsy_moth Gypsy moth]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UM9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UM9 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6um9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6um9 OCA], [http://pdbe.org/6um9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6um9 RCSB], [http://www.ebi.ac.uk/pdbsum/6um9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6um9 ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PBP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=13123 Gypsy moth])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6um9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6um9 OCA], [http://pdbe.org/6um9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6um9 RCSB], [http://www.ebi.ac.uk/pdbsum/6um9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6um9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pheromone-binding proteins (PBPs) are small, water-soluble proteins found in the lymph of pheromone-sensing hairs. PBPs are essential in modulating pheromone partitioning in the lymph and at pheromone receptors of olfactory sensory neurons. The function of a PBP is associated with its ability to structurally convert between two conformations. Although mechanistic details remain unclear, it has been proposed that the structural transition between these forms is affected by two factors: pH and the presence or absence of ligand. To better understand the PBP conformational transition, the structure of the gypsy moth (Lymantria dispar) LdisPBP1 was elucidated at pH 4.5 and 35 degrees C using nuclear magnetic resonance spectroscopy. In addition, the effects of sample pH and binding of the species' pheromone, (+)-disparlure, (7R,8S)-epoxy-2-methyloctadecane, and its enantiomer were monitored via (15)N HSQC spectroscopy. LdisPBP1 in acidic conditions has seven helices, with its C-terminal residues forming the seventh helix within the pheromone-binding pocket and its N-terminal residues disordered. Under conditions where this conformation is made favorable, free LdisPBP1 would have limited ligand binding capacity due to the seventh helix occupying the internal binding pocket. Our findings suggest that even in the presence of 4-fold ligand at acidic pH, LdisPBP1 is only approximately 60% in its pheromone-bound form. Furthermore, evidence of a different LdisPBP1 form is seen at higher pH, with the transition pH between 5.6 and 6.0. This suggests that LdisPBP1 at neutral pH exists as a mixture of at least two conformations. These findings have implications concerning the PBP ligand binding and release mechanism. | ||
| + | |||
| + | Ligand- and pH-Induced Structural Transition of Gypsy Moth Lymantria dispar Pheromone-Binding Protein 1 (LdisPBP1).,Terrado M, Okon M, McIntosh LP, Plettner E Biochemistry. 2020 Sep 22;59(37):3411-3426. doi: 10.1021/acs.biochem.0c00592., Epub 2020 Sep 11. PMID:32877603<ref>PMID:32877603</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6um9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Gypsy moth]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Plettner E]] | + | [[Category: Plettner, E]] |
| - | [[Category: Terrado M]] | + | [[Category: Terrado, M]] |
| + | [[Category: Lipid binding protein]] | ||
Revision as of 06:37, 7 October 2020
Gypsy Moth Pheromone-binding protein 1 (LdisPBP1) NMR Structure at pH 4.5
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