6xmp
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Structure of P5A-ATPase Spf1, Apo form== | ==Structure of P5A-ATPase Spf1, Apo form== | ||
| - | <StructureSection load='6xmp' size='340' side='right'caption='[[6xmp]]' scene=''> | + | <StructureSection load='6xmp' size='340' side='right'caption='[[6xmp]], [[Resolution|resolution]] 3.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XMP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XMP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xmp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XMP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XMP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xmp OCA], [http://pdbe.org/6xmp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xmp RCSB], [http://www.ebi.ac.uk/pdbsum/6xmp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xmp ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xmp OCA], [http://pdbe.org/6xmp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xmp RCSB], [http://www.ebi.ac.uk/pdbsum/6xmp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xmp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/ATC6_YEAST ATC6_YEAST]] Mediates manganese transport into the endoplasmic reticulum. The ATPase activity is required for cellular manganese homeostasis.<ref>PMID:24392018</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Organelle identity depends on protein composition. How mistargeted proteins are selectively recognized and removed from organelles is incompletely understood. Here, we found that the orphan P5A-adenosine triphosphatase (ATPase) transporter ATP13A1 (Spf1 in yeast) directly interacted with the transmembrane segment (TM) of mitochondrial tail-anchored proteins. P5A-ATPase activity mediated the extraction of mistargeted proteins from the endoplasmic reticulum (ER). Cryo-electron microscopy structures of Saccharomyces cerevisiae Spf1 revealed a large, membrane-accessible substrate-binding pocket that alternately faced the ER lumen and cytosol and an endogenous substrate resembling an alpha-helical TM. Our results indicate that the P5A-ATPase could dislocate misinserted hydrophobic helices flanked by short basic segments from the ER. TM dislocation by the P5A-ATPase establishes an additional class of P-type ATPase substrates and may correct mistakes in protein targeting or topogenesis. | ||
| + | |||
| + | The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.,McKenna MJ, Sim SI, Ordureau A, Wei L, Harper JW, Shao S, Park E Science. 2020 Sep 25;369(6511). pii: 369/6511/eabc5809. doi:, 10.1126/science.abc5809. PMID:32973005<ref>PMID:32973005</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6xmp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Baker's yeast]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Park E]] | + | [[Category: Park, E]] |
| - | [[Category: Sim | + | [[Category: Sim, S I]] |
| + | [[Category: Endoplasmic reticulum]] | ||
| + | [[Category: P-type atpase]] | ||
| + | [[Category: Protein quality control]] | ||
| + | [[Category: Transmembrane helix dislocase]] | ||
| + | [[Category: Transport protein]] | ||
Revision as of 06:40, 7 October 2020
Structure of P5A-ATPase Spf1, Apo form
| |||||||||||
