This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2y6t

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 06:58, 7 October 2020

Molecular Recognition of Chymotrypsin by the Serine Protease Inhibitor Ecotin from Yersinia pestis

Structural highlights

2y6t is a 8 chain structure with sequence from Bos taurus and Yerpy. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2gct, 4vgc, 1gcd, 1mtn, 1gmh, 8gch, 1t8n, 1gct, 1gg6, 1t8o, 3gct, 5cha, 1p2q, 1ab9, 1gl1, 2vgc, 2cga, 1cgj, 1cho, 1cbw, 2gmt, 1ghb, 6gch, 1k2i, 1gha, 3vgc, 7gch, 3gch, 1cgi, 4gch, 1gmc, 1ca0, 1afq, 1oxg, 1gl0, 1chg, 1ggd, 2gch, 1t7c, 1yph, 1p2o, 1dlk, 1p2n, 1n8o, 1t8m, 1gmd, 4cha, 5gch, 1ex3, 1p2m, 1vgc, 1t8l, 2cha, 1hja, 6cha, 1acb
Activity:	Chymotrypsin, with EC number 3.4.21.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ECOT_YERPY] General inhibitor of pancreatic serine proteases: inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases (By similarity).

Publication Abstract from PubMed

Resistance to antibiotics is a problem not only in terms of healthcare but also biodefense. Engineering of resistance into a human pathogen could create an untreatable biothreat pathogen. One such pathogen is Yersinia pestis, the causative agent of plague. Previously, we have used a bioinformatic approach to identify proteins that may be suitable targets for antimicrobial therapy and in particular for the treatment of plague. The serine protease inhibitor ecotin was identified as one such target. We have carried out mutational analyses in the closely related Yersinia pseudotuberculosis, validating that the ecotin gene is a virulence-associated gene in this bacterium. Y. pestis ecotin inhibits chymotrypsin. Here, we present the structure of ecotin in complex with chymotrypsin to 2.74 A resolution. The structure features a biologically relevant tetramer whereby an ecotin dimer binds to two chymotrypsin molecules, similar to what was observed in related serine protease inhibitor structures. However, the vast majority of the interactions in the present structure are distinctive, indicating that the broad specificity of the inhibitor for these proteases is based largely on its capacity to recognize features unique to each of them. These findings will have implications for the development of small ecotin inhibitors for therapeutic use.

Molecular Recognition of Chymotrypsin by the Serine Protease Inhibitor Ecotin from Yersinia pestis.,Clark EA, Walker N, Ford DC, Cooper IA, Oyston PC, Acharya KR J Biol Chem. 2011 Jul 8;286(27):24015-22. Epub 2011 Apr 29. PMID:21531711^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Clark EA, Walker N, Ford DC, Cooper IA, Oyston PC, Acharya KR. Molecular Recognition of Chymotrypsin by the Serine Protease Inhibitor Ecotin from Yersinia pestis. J Biol Chem. 2011 Jul 8;286(27):24015-22. Epub 2011 Apr 29. PMID:21531711 doi:10.1074/jbc.M111.225730

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2y6t"

@@ Line 1: / Line 1: @@
 ==Molecular Recognition of Chymotrypsin by the Serine Protease Inhibitor Ecotin from Yersinia pestis==
-<StructureSection load='2y6t' size='340' side='right' caption='[[2y6t]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
+<StructureSection load='2y6t' size='340' side='right'caption='[[2y6t]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2y6t]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Yerpy Yerpy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y6T FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2y6t]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Yerpy Yerpy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y6T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2Y6T FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gct|2gct]], [[4vgc|4vgc]], [[1gcd|1gcd]], [[1mtn|1mtn]], [[1gmh|1gmh]], [[8gch|8gch]], [[1t8n|1t8n]], [[1gct|1gct]], [[1gg6|1gg6]], [[1t8o|1t8o]], [[3gct|3gct]], [[5cha|5cha]], [[1p2q|1p2q]], [[1ab9|1ab9]], [[1gl1|1gl1]], [[2vgc|2vgc]], [[2cga|2cga]], [[1cgj|1cgj]], [[1cho|1cho]], [[1cbw|1cbw]], [[2gmt|2gmt]], [[1ghb|1ghb]], [[6gch|6gch]], [[1k2i|1k2i]], [[1gha|1gha]], [[3vgc|3vgc]], [[7gch|7gch]], [[3gch|3gch]], [[1cgi|1cgi]], [[4gch|4gch]], [[1gmc|1gmc]], [[1ca0|1ca0]], [[1afq|1afq]], [[1oxg|1oxg]], [[1gl0|1gl0]], [[1chg|1chg]], [[1ggd|1ggd]], [[2gch|2gch]], [[1t7c|1t7c]], [[1yph|1yph]], [[1p2o|1p2o]], [[1dlk|1dlk]], [[1p2n|1p2n]], [[1n8o|1n8o]], [[1t8m|1t8m]], [[1gmd|1gmd]], [[4cha|4cha]], [[5gch|5gch]], [[1ex3|1ex3]], [[1p2m|1p2m]], [[1vgc|1vgc]], [[1t8l|1t8l]], [[2cha|2cha]], [[1hja|1hja]], [[6cha|6cha]], [[1acb|1acb]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y6t OCA], [http://pdbe.org/2y6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y6t RCSB], [http://www.ebi.ac.uk/pdbsum/2y6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y6t ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2y6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y6t OCA], [http://pdbe.org/2y6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y6t RCSB], [http://www.ebi.ac.uk/pdbsum/2y6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y6t ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 ==See Also==
-*[[Chymotrypsin|Chymotrypsin]]
+*[[Chymotrypsin 3D structures|Chymotrypsin 3D structures]]
 *[[Ecotin|Ecotin]]
 == References ==
@@ Line 30: / Line 30: @@
 [[Category: Bos taurus]]
 [[Category: Chymotrypsin]]
+[[Category: Large Structures]]
 [[Category: Yerpy]]
 [[Category: Acharya, K R]]

2y6t

From Proteopedia

Revision as of 06:58, 7 October 2020

Molecular Recognition of Chymotrypsin by the Serine Protease Inhibitor Ecotin from Yersinia pestis

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox