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3k7z

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Revision as of 07:00, 7 October 2020

GCN4-Leucine zipper core mutant as N16A trigonal automatic solution

Structural highlights

3k7z is a 3 chain structure. This structure supersedes the now removed PDB entry 1rb1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1rb4, 1rb5, 1rb6
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.

Publication Abstract from PubMed

Efficient determination of protein crystal structures requires automated x-ray data analysis. Here, we describe the expert system ELVES and its use to determine automatically the structure of a 12-kDa protein. Multiwavelength anomalous diffraction analysis of a selenomethionyl derivative was used to image the Asn-16-Ala variant of the GCN4 leucine zipper. In contrast to the parallel, dimeric coiled coil formed by the WT sequence, the mutant unexpectedly formed an antiparallel trimer. This structural switch reveals how avoidance of core cavities at a single site can select the native fold of a protein. All structure calculations, including indexing, data processing, locating heavy atoms, phasing by multiwavelength anomalous diffraction, model building, and refinement, were completed without human intervention. The results demonstrate the feasibility of automated methods for determining high-resolution, x-ray crystal structures of proteins.

Automated protein crystal structure determination using ELVES.,Holton J, Alber T Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1537-42. Epub 2004 Jan 29. PMID:14752198^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Holton J, Alber T. Automated protein crystal structure determination using ELVES. Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1537-42. Epub 2004 Jan 29. PMID:14752198 doi:10.1073/pnas.0306241101

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3k7z"

@@ Line 1: / Line 1: @@
 ==GCN4-Leucine zipper core mutant as N16A trigonal automatic solution==
-<StructureSection load='3k7z' size='340' side='right' caption='[[3k7z]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='3k7z' size='340' side='right'caption='[[3k7z]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rb4|1rb4]], [[1rb5|1rb5]], [[1rb6|1rb6]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [http://pdbe.org/3k7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [http://www.ebi.ac.uk/pdbsum/3k7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7z ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [http://pdbe.org/3k7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [http://www.ebi.ac.uk/pdbsum/3k7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7z ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 20: / Line 20: @@
 ==See Also==
-*[[Gcn4|Gcn4]]
+*[[Gnc4 3D Structures|Gnc4 3D Structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Alber, T]]
 [[Category: Holton, J]]

3k7z

From Proteopedia

Revision as of 07:00, 7 October 2020

GCN4-Leucine zipper core mutant as N16A trigonal automatic solution

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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