1cru

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[[Image:1cru.gif|left|200px]]
[[Image:1cru.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1cru |SIZE=350|CAPTION= <scene name='initialview01'>1cru</scene>, resolution 1.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1cru", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HDN:METHYLHYDRAZINE'>HDN</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Quinoprotein_glucose_dehydrogenase Quinoprotein glucose dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.5.2 1.1.5.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1cru| PDB=1cru | SCENE= }}
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|RELATEDENTRY=[[1qbi|1QBI]], [[1c9u|1C9U]], [[1cq1|1CQ1]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cru OCA], [http://www.ebi.ac.uk/pdbsum/1cru PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cru RCSB]</span>
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}}
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'''SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE FROM ACINETOBACTER CALCOACETICUS IN COMPLEX WITH PQQ AND METHYLHYDRAZINE'''
'''SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE FROM ACINETOBACTER CALCOACETICUS IN COMPLEX WITH PQQ AND METHYLHYDRAZINE'''
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[[Category: Oubrie, A.]]
[[Category: Oubrie, A.]]
[[Category: Rozeboom, H J.]]
[[Category: Rozeboom, H J.]]
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[[Category: beta-propeller]]
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[[Category: Beta-propeller]]
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[[Category: complex with the cofactor pqq and the inhibitor methylhydrazine]]
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[[Category: Complex with the cofactor pqq and the inhibitor methylhydrazine]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: superbarrel]]
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[[Category: Superbarrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:02:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:27:22 2008''
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Revision as of 10:02, 2 May 2008

Image:1cru.gif

Template:STRUCTURE 1cru

SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE FROM ACINETOBACTER CALCOACETICUS IN COMPLEX WITH PQQ AND METHYLHYDRAZINE


Overview

Soluble glucose dehydrogenase (s-GDH) from the bacterium Acinetobacter calcoaceticus is a classical quinoprotein. It requires the cofactor pyrroloquinoline quinone (PQQ) to catalyze the oxidation of glucose to gluconolactone. The precise catalytic role of PQQ in s-GDH and several other PQQ-dependent enzymes has remained controversial because of the absence of comprehensive structural data. We have determined the crystal structure of a ternary complex of s-GDH with PQQ and methylhydrazine, a competitive inhibitor of the enzyme. This complex, refined at 1.5-A resolution to an R factor of 16.7%, affords a detailed view of a cofactor-binding site of s-GDH. Moreover, it presents the first direct observation of covalent PQQ adduct in the active-site of a PQQ-dependent enzyme, thereby confirming previous evidence that the C5 carbonyl group of the cofactor is the most reactive moiety of PQQ.

About this Structure

1CRU is a Single protein structure of sequence from Acinetobacter calcoaceticus. Full crystallographic information is available from OCA.

Reference

Active-site structure of the soluble quinoprotein glucose dehydrogenase complexed with methylhydrazine: a covalent cofactor-inhibitor complex., Oubrie A, Rozeboom HJ, Dijkstra BW, Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11787-91. PMID:10518528 Page seeded by OCA on Fri May 2 13:02:59 2008

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