3l0o

From Proteopedia

(Difference between revisions)

Revision as of 07:04, 7 October 2020

Structure of RNA-free Rho transcription termination factor from Thermotoga maritima

Structural highlights

3l0o is a 2 chain structure with sequence from Atcc 43589. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RHO_THEMA] Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.[HAMAP-Rule:MF_01884]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The Rho factor is a ring-shaped ATP-dependent helicase that mediates transcription termination in most prokaryotic cells by disengaging the transcription elongation complex formed by the RNA polymerase, DNA, and the nascent RNA transcript. The crystal structures of key intermediates along the kinetic pathway of RNA binding to Rho unveiled an unprecedented mode of helicase loading and provided a model for the ATP turnover coupled to coordinated strand movement. Here we report the structure of the early RNA-free state of Rho, which had eluded crystallization for many years but now completes the series. The structure allows the characterization of the apo-form Rho from Thermotoga maritima to 2.3 A resolution, reveals an RNA-recruiting site that becomes hidden after occupancy of the adjacent specific primary RNA-binding site, and suggests an enriched model for mRNA capture that is consistent with previous data.

The structure of RNA-free Rho termination factor indicates a dynamic mechanism of transcript capture.,Canals A, Uson I, Coll M J Mol Biol. 2010 Jul 2;400(1):16-23. Epub 2010 May 7. PMID:20452362^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Canals A, Uson I, Coll M. The structure of RNA-free Rho termination factor indicates a dynamic mechanism of transcript capture. J Mol Biol. 2010 Jul 2;400(1):16-23. Epub 2010 May 7. PMID:20452362 doi:10.1016/j.jmb.2010.05.004

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3l0o"

@@ Line 1: / Line 1: @@
 ==Structure of RNA-free Rho transcription termination factor from Thermotoga maritima==
-<StructureSection load='3l0o' size='340' side='right' caption='[[3l0o]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
+<StructureSection load='3l0o' size='340' side='right'caption='[[3l0o]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3l0o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L0O FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3l0o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L0O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3L0O FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l0o OCA], [http://pdbe.org/3l0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l0o RCSB], [http://www.ebi.ac.uk/pdbsum/3l0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l0o ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3l0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l0o OCA], [http://pdbe.org/3l0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l0o RCSB], [http://www.ebi.ac.uk/pdbsum/3l0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l0o ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Helicase|Helicase]]
+*[[Helicase 3D structures|Helicase 3D structures]]
 == References ==
 <references/>
@@ Line 36: / Line 36: @@
 </StructureSection>
 [[Category: Atcc 43589]]
+[[Category: Large Structures]]
 [[Category: Canals, A]]
 [[Category: Coll, M]]

3l0o

From Proteopedia

Revision as of 07:04, 7 October 2020

Structure of RNA-free Rho transcription termination factor from Thermotoga maritima

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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