1crx

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[[Image:1crx.gif|left|200px]]
[[Image:1crx.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1crx |SIZE=350|CAPTION= <scene name='initialview01'>1crx</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1crx", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= CRE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10678 Enterobacteria phage P1])
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|DOMAIN=
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{{STRUCTURE_1crx| PDB=1crx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crx OCA], [http://www.ebi.ac.uk/pdbsum/1crx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1crx RCSB]</span>
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'''CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I'''
'''CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I'''
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[[Category: Gopaul, D N.]]
[[Category: Gopaul, D N.]]
[[Category: Guo, F.]]
[[Category: Guo, F.]]
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[[Category: complex (recombinase/dna)]]
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[[Category: Protein:dna complex]]
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[[Category: protein:dna complex]]
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[[Category: Reaction intermediate]]
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[[Category: reaction intermediate]]
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[[Category: Site-specific recombinase]]
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[[Category: site-specific recombinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:03:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:27:27 2008''
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Revision as of 10:03, 2 May 2008

Image:1crx.gif


PDB ID 1crx

Drag the structure with the mouse to rotate
1crx, resolution 2.40Å ()
Ligands:
Gene: CRE (Enterobacteria phage P1)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I


Overview

During site-specific DNA recombination, which brings about genetic rearrangement in processes such as viral integration and excision and chromosomal segregation, recombinase enzymes recognize specific DNA sequences and catalyse the reciprocal exchange of DNA strands between these sites. The bacteriophage recombinase Cre catalyses site-specific recombination between two 34-base-pair loxP sites. The crystal structure at 2.4 A resolution of Cre bound to a loxP substrate reveals an intermediate in the recombination reaction, in which a Cre molecule has cleaved the substrate to form a covalent 3'-phosphotyrosine linkage with the DNA. Four recombinases and two loxP sites form a synapsed structure in which the DNA resembles models of four-way Holliday-Junction intermediates. The Cre-loxP complex challenges models of site-specific recombination that require large changes in quaternary structure. Subtle allosteric changes at the carboxy termini of the Cre subunits may instead coordinate the cleavage and strand-exchange reactions.

About this Structure

1CRX is a Single protein structure of sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.

Reference

Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse., Guo F, Gopaul DN, van Duyne GD, Nature. 1997 Sep 4;389(6646):40-6. PMID:9288963 Page seeded by OCA on Fri May 2 13:03:10 2008

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