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5wrs

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Revision as of 07:15, 7 October 2020

Crystal Structure of Fam20A in complex with ATP

Structural highlights

5wrs is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	5wrr
Gene:	FAM20A, UNQ9388/PRO34279 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[FA20A_HUMAN] Amelogenesis imperfecta - nephrocalcinosis;Amelogenesis imperfecta and gingival hyperplasia syndrome. The disease is caused by mutations affecting the gene represented in this entry.

Function

[FA20A_HUMAN] Pseudokinase that acts as an allosteric activator of the Golgi serine/threonine protein kinase FAM20C and is involved in biomineralization of teeth. Forms a complex with FAM20C and increases the ability of FAM20C to phosphorylate the proteins that form the 'matrix' that guides the deposition of the enamel minerals.^[1]

Publication Abstract from PubMed

Mutations in FAM20A cause tooth enamel defects known as Amelogenesis Imperfecta (AI) and renal calcification. We previously showed that Fam20A is a secretory pathway pseudokinase and allosterically activates the physiological casein kinase Fam20C to phosphorylate secreted proteins important for biomineralization (Cui et al., 2015). Here we report the nucleotide-free and ATP-bound structures of Fam20A. Fam20A exhibits a distinct disulfide bond pattern mediated by a unique insertion region. Loss of this insertion due to abnormal mRNA splicing interferes with the structure and function of Fam20A, resulting in AI. Fam20A binds ATP in the absence of divalent cations, and strikingly, ATP is bound in an inverted orientation compared to other kinases. Fam20A forms a dimer in the crystal, and residues in the dimer interface are critical for Fam20C activation. Together, these results provide structural insights into the function of Fam20A and shed light on the mechanism by which Fam20A mutations cause disease.

Structure of Fam20A reveals a pseudokinase featuring a unique disulfide pattern and inverted ATP-binding.,Cui J, Zhu Q, Zhang H, Cianfrocco MA, Leschziner AE, Dixon JE, Xiao J Elife. 2017 Apr 22;6. pii: e23990. doi: 10.7554/eLife.23990. PMID:28432788^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cui J, Xiao J, Tagliabracci VS, Wen J, Rahdar M, Dixon JE. A secretory kinase complex regulates extracellular protein phosphorylation. Elife. 2015 Mar 19;4:e06120. doi: 10.7554/eLife.06120. PMID:25789606 doi:http://dx.doi.org/10.7554/eLife.06120
↑ Cui J, Zhu Q, Zhang H, Cianfrocco MA, Leschziner AE, Dixon JE, Xiao J. Structure of Fam20A reveals a pseudokinase featuring a unique disulfide pattern and inverted ATP-binding. Elife. 2017 Apr 22;6. pii: e23990. doi: 10.7554/eLife.23990. PMID:28432788 doi:http://dx.doi.org/10.7554/eLife.23990

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wrs"

Categories: Human | Large Structures | Zhu, Q | Secretory pathway pseudokinase | Transferase

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Fam20A in complex with ATP==
-<StructureSection load='5wrs' size='340' side='right' caption='[[5wrs]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
+<StructureSection load='5wrs' size='340' side='right'caption='[[5wrs]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wrs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WRS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WRS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wrs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WRS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WRS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wrr|5wrr]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wrs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wrs OCA], [http://pdbe.org/5wrs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wrs RCSB], [http://www.ebi.ac.uk/pdbsum/5wrs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wrs ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FAM20A, UNQ9388/PRO34279 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wrs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wrs OCA], [http://pdbe.org/5wrs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wrs RCSB], [http://www.ebi.ac.uk/pdbsum/5wrs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wrs ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 25: / Line 26: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Zhu, Q]]
 [[Category: Secretory pathway pseudokinase]]
 [[Category: Transferase]]

5wrs

From Proteopedia

Revision as of 07:15, 7 October 2020

Crystal Structure of Fam20A in complex with ATP

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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