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5wty

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Revision as of 07:17, 7 October 2020

Structure of Nop9 RNA complex

Structural highlights

5wty is a 4 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5wtx
Gene:	NOP9 (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NOP9_YEAST] RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.^[1] ^[2]

Publication Abstract from PubMed

The assembly of eukaryotic ribosomes requires numerous factors that transiently associate with evolving pre-ribosomal particles. The Pumilio repeat-containing protein Nop9 briefly associates with the 90S pre-ribosome during its co-transcriptional assembly. Here, we show that Nop9 specifically binds an 11-nucleotide sequence of 18S rRNA that forms the 3' side of the central pseudoknot and helix 28 in the mature subunit. Crystal structures of Nop9 in the free and RNA-bound states reveal a new type of Pumilio repeat protein with a distinct structure, target sequence and RNA-binding mode. Nop9 contains 10 Pumilio repeats arranged into a U-shaped scaffold. The target RNA is recognized by two stretches of repeats in a bipartite manner, and three central bases are unrecognized as a result of the degeneracy of repeats 6 and 7. Our data suggest that Nop9 regulates the folding of 18S rRNA at early assembly stages of 90S.

Nop9 binds the central pseudoknot region of 18S rRNA.,Wang B, Ye K Nucleic Acids Res. 2017 Jan 3. pii: gkw1323. doi: 10.1093/nar/gkw1323. PMID:28053123^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wade CH, Umbarger MA, McAlear MA. The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes. Yeast. 2006 Mar;23(4):293-306. PMID:16544271 doi:http://dx.doi.org/10.1002/yea.1353
↑ Thomson E, Rappsilber J, Tollervey D. Nop9 is an RNA binding protein present in pre-40S ribosomes and required for 18S rRNA synthesis in yeast. RNA. 2007 Dec;13(12):2165-74. Epub 2007 Oct 23. PMID:17956976 doi:http://dx.doi.org/10.1261/rna.747607
↑ Wang B, Ye K. Nop9 binds the central pseudoknot region of 18S rRNA. Nucleic Acids Res. 2017 Jan 3. pii: gkw1323. doi: 10.1093/nar/gkw1323. PMID:28053123 doi:http://dx.doi.org/10.1093/nar/gkw1323

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wty"

@@ Line 1: / Line 1: @@
 ==Structure of Nop9 RNA complex==
-<StructureSection load='5wty' size='340' side='right' caption='[[5wty]], [[Resolution|resolution]] 2.79&Aring;' scene=''>
+<StructureSection load='5wty' size='340' side='right'caption='[[5wty]], [[Resolution|resolution]] 2.79&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wty]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WTY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wty]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WTY FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wtx|5wtx]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wty OCA], [http://pdbe.org/5wty PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wty RCSB], [http://www.ebi.ac.uk/pdbsum/5wty PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wty ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NOP9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wty OCA], [http://pdbe.org/5wty PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wty RCSB], [http://www.ebi.ac.uk/pdbsum/5wty PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wty ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
+[[Category: Large Structures]]
 [[Category: Wang, B]]
 [[Category: Ye, K]]

5wty

From Proteopedia

Revision as of 07:17, 7 October 2020

Structure of Nop9 RNA complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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