5x3p

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the UBX domain of human UBXD7

Structural highlights

5x3p is a 3 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	5x3s
Gene:	UBXN7, KIAA0794, UBXD7 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[UBXN7_HUMAN] Ubiquitin-binding adapter that links a subset of NEDD8-associated cullin ring ligases (CRLs) to the segregase VCP/p97, to regulate turnover of their ubiquitination substrates.^[1]

Publication Abstract from PubMed

In humans, UBXD7 (also called UBXN7), an adaptor of p97 ATPase, can participate in the degradation of misfolded or damaged proteins in the p97-mediated ubiquitin proteasome system (UPS). UBXD7 binds to ubiquitinated substrates via its UBA domain and interacts with p97 N-terminal domain through its UBX domain to recruit p97 or the p97 core complex (p97/NPL4/UFD1). Here, we report the crystal structures of the UBX domain of UBXD7 (UBXD7UBX) at 2.0 A resolution and its complex with p97 N-terminal domain (p97NTD-UBXD7UBX complex) at 2.4 A resolution. A structural analysis and isothermal titration calorimetry results provide detailed molecular basis of interaction between UBXD7UBX and p97NTD. Moreover, structural superpositions suggest that dimerization of UBXD7UBX via an intermolecular disulfide bond could interfere with the formation of the p97NTD-UBXD7UBX complex. Interestingly, UBXD7 may have a cooperative effect on p97 interaction with UFD1. Together, these results provide structural and biochemical insights into the interaction between p97NTD and UBXD7UBX.

Crystal structures of the UBX domain of human UBXD7 and its complex with p97 ATPase.,Li ZH, Wang Y, Xu M, Jiang T Biochem Biophys Res Commun. 2017 Mar 6. pii: S0006-291X(17)30444-8. doi:, 10.1016/j.bbrc.2017.03.005. PMID:28274878^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bandau S, Knebel A, Gage ZO, Wood NT, Alexandru G. UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1alpha accumulation. BMC Biol. 2012 Apr 26;10:36. doi: 10.1186/1741-7007-10-36. PMID:22537386 doi:http://dx.doi.org/10.1186/1741-7007-10-36
↑ Li ZH, Wang Y, Xu M, Jiang T. Crystal structures of the UBX domain of human UBXD7 and its complex with p97 ATPase. Biochem Biophys Res Commun. 2017 Mar 6. pii: S0006-291X(17)30444-8. doi:, 10.1016/j.bbrc.2017.03.005. PMID:28274878 doi:http://dx.doi.org/10.1016/j.bbrc.2017.03.005

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5x3p"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the UBX domain of human UBXD7==
-<StructureSection load='5x3p' size='340' side='right' caption='[[5x3p]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='5x3p' size='340' side='right'caption='[[5x3p]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5x3p]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X3P FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5x3p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X3P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X3P FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5x3s|5x3s]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x3p OCA], [http://pdbe.org/5x3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x3p RCSB], [http://www.ebi.ac.uk/pdbsum/5x3p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x3p ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UBXN7, KIAA0794, UBXD7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x3p OCA], [http://pdbe.org/5x3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x3p RCSB], [http://www.ebi.ac.uk/pdbsum/5x3p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x3p ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Jiang, T]]
 [[Category: Li, Z]]

5x3p

From Proteopedia

Current revision

Crystal structure of the UBX domain of human UBXD7

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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