This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5x41

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:24, 7 October 2020

3.5A resolution structure of a cobalt energy-coupling factor transporter using LCP method-CbiMQO

Structural highlights

5x41 is a 8 chain structure with sequence from "rhodonostoc_capsulatum"_molisch_1907 "rhodonostoc capsulatum" molisch 1907. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5x3x, 5x40
Gene:	cbiM ("Rhodonostoc capsulatum" Molisch 1907)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[A0A0Q0R232_RHOCA] Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.[HAMAP-Rule:MF_01462]

Publication Abstract from PubMed

Energy-coupling factor (ECF) transporters are a large family of ATP-binding cassette transporters recently identified in microorganisms. Responsible for micronutrient uptake from the environment, ECF transporters are modular transporters composed of a membrane substrate-binding component EcfS and an ECF module consisting of an integral membrane scaffold component EcfT and two cytoplasmic ATP binding/hydrolysis components EcfA/A'. ECF transporters are classified into groups I and II. Currently, the molecular understanding of group-I ECF transporters is very limited, partly due to a lack of transporter complex structural information. Here, we present structures and structure-based analyses of the group-I cobalt ECF transporter CbiMNQO, whose constituting subunits CbiM/CbiN, CbiQ, and CbiO correspond to the EcfS, EcfT, and EcfA components of group-II ECF transporters, respectively. Through reconstitution of different CbiMNQO subunits and determination of related ATPase and transporter activities, the substrate-binding subunit CbiM was found to stimulate CbiQO's basal ATPase activity. The structure of CbiMQO complex was determined in its inward-open conformation and that of CbiO in beta, gamma-methyleneadenosine 5'-triphosphate-bound closed conformation. Structure-based analyses revealed interactions between different components, substrate-gating function of the L1 loop of CbiM, and conformational changes of CbiO induced by ATP binding and product release within the CbiMNQO transporter complex. These findings enabled us to propose a working model of the CbiMNQO transporter, in which the transport process requires the rotation or toppling of both CbiQ and CbiM, and CbiN might function in coupling conformational changes between CbiQ and CbiM.Cell Research advance online publication 21 March 2017; doi:10.1038/cr.2017.38.

Structure and mechanism of a group-I cobalt energy coupling factor transporter.,Bao Z, Qi X, Hong S, Xu K, He F, Zhang M, Chen J, Chao D, Zhao W, Li D, Wang J, Zhang P Cell Res. 2017 Mar 21. doi: 10.1038/cr.2017.38. PMID:28322252^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bao Z, Qi X, Hong S, Xu K, He F, Zhang M, Chen J, Chao D, Zhao W, Li D, Wang J, Zhang P. Structure and mechanism of a group-I cobalt energy coupling factor transporter. Cell Res. 2017 Mar 21. doi: 10.1038/cr.2017.38. PMID:28322252 doi:http://dx.doi.org/10.1038/cr.2017.38

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5x41"

@@ Line 1: / Line 1: @@
 ==3.5A resolution structure of a cobalt energy-coupling factor transporter using LCP method-CbiMQO==
-<StructureSection load='5x41' size='340' side='right' caption='[[5x41]], [[Resolution|resolution]] 3.47&Aring;' scene=''>
+<StructureSection load='5x41' size='340' side='right'caption='[[5x41]], [[Resolution|resolution]] 3.47&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5x41]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X41 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X41 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5x41]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodonostoc_capsulatum"_molisch_1907 "rhodonostoc capsulatum" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X41 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X41 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5x3x|5x3x]], [[5x40|5x40]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x41 OCA], [http://pdbe.org/5x41 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x41 RCSB], [http://www.ebi.ac.uk/pdbsum/5x41 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x41 ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cbiM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1061 "Rhodonostoc capsulatum" Molisch 1907])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x41 OCA], [http://pdbe.org/5x41 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x41 RCSB], [http://www.ebi.ac.uk/pdbsum/5x41 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x41 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Rhodonostoc capsulatum molisch 1907]]
+[[Category: Large Structures]]
 [[Category: Bao, Z]]
 [[Category: Li, D]]

5x41

From Proteopedia

Revision as of 07:24, 7 October 2020

3.5A resolution structure of a cobalt energy-coupling factor transporter using LCP method-CbiMQO

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox