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5x57

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Revision as of 07:26, 7 October 2020

Structure of GAR domain of ACF7

Structural highlights

5x57 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MACF1_HUMAN] Isoform 2 is a F-actin-binding protein which may play a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane. Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics. May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4. Plays a key role in wound healing and epidermal cell migration. Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate MT dynamics and polarize hair follicle stem cells (By similarity).^[1] ^[2]

Publication Abstract from PubMed

In the intestinal epithelium, the aberrant regulation of cell/cell junctions leads to intestinal barrier defects, which may promote the onset and enhance the severity of inflammatory bowel disease (IBD). However, it remains unclear how the coordinated behaviour of cytoskeletal network may contribute to cell junctional dynamics. In this report, we identified ACF7, a crosslinker of microtubules and F-actin, as an essential player in this process. Loss of ACF7 leads to aberrant microtubule organization, tight junction stabilization and impaired wound closure in vitro. With the mouse genetics approach, we show that ablation of ACF7 inhibits intestinal wound healing and greatly increases susceptibility to experimental colitis in mice. ACF7 level is also correlated with development and progression of ulcerative colitis (UC) in human patients. Together, our results reveal an important molecular mechanism whereby coordinated cytoskeletal dynamics contributes to cell adhesion regulation during intestinal wound repair and the development of IBD.

ACF7 regulates inflammatory colitis and intestinal wound response by orchestrating tight junction dynamics.,Ma Y, Yue J, Zhang Y, Shi C, Odenwald M, Liang WG, Wei Q, Goel A, Gou X, Zhang J, Chen SY, Tang WJ, Turner JR, Yang F, Liang H, Qin H, Wu X Nat Commun. 2017 May 25;8:15375. doi: 10.1038/ncomms15375. PMID:28541346^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kakinuma T, Ichikawa H, Tsukada Y, Nakamura T, Toh BH. Interaction between p230 and MACF1 is associated with transport of a glycosyl phosphatidyl inositol-anchored protein from the Golgi to the cell periphery. Exp Cell Res. 2004 Aug 15;298(2):388-98. PMID:15265687 doi:http://dx.doi.org/10.1016/j.yexcr.2004.04.047
↑ Zaoui K, Benseddik K, Daou P, Salaun D, Badache A. ErbB2 receptor controls microtubule capture by recruiting ACF7 to the plasma membrane of migrating cells. Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18517-22. doi:, 10.1073/pnas.1000975107. Epub 2010 Oct 11. PMID:20937854 doi:10.1073/pnas.1000975107
↑ Ma Y, Yue J, Zhang Y, Shi C, Odenwald M, Liang WG, Wei Q, Goel A, Gou X, Zhang J, Chen SY, Tang WJ, Turner JR, Yang F, Liang H, Qin H, Wu X. ACF7 regulates inflammatory colitis and intestinal wound response by orchestrating tight junction dynamics. Nat Commun. 2017 May 25;8:15375. doi: 10.1038/ncomms15375. PMID:28541346 doi:http://dx.doi.org/10.1038/ncomms15375

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5x57"

@@ Line 1: / Line 1: @@
 ==Structure of GAR domain of ACF7==
-<StructureSection load='5x57' size='340' side='right' caption='[[5x57]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
+<StructureSection load='5x57' size='340' side='right'caption='[[5x57]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5x57]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X57 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X57 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5x57]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X57 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X57 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x57 OCA], [http://pdbe.org/5x57 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x57 RCSB], [http://www.ebi.ac.uk/pdbsum/5x57 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x57 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x57 OCA], [http://pdbe.org/5x57 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x57 RCSB], [http://www.ebi.ac.uk/pdbsum/5x57 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x57 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Wang, T]]
 [[Category: Wu, X Y]]

5x57

From Proteopedia

Revision as of 07:26, 7 October 2020

Structure of GAR domain of ACF7

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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