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6sbd
From Proteopedia
(Difference between revisions)
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==Structure of type II terpene cyclase MstE_D109A from Scytonema in complex with merosterolic acid A (product)== | ==Structure of type II terpene cyclase MstE_D109A from Scytonema in complex with merosterolic acid A (product)== | ||
| - | <StructureSection load='6sbd' size='340' side='right'caption='[[6sbd]]' scene=''> | + | <StructureSection load='6sbd' size='340' side='right'caption='[[6sbd]], [[Resolution|resolution]] 1.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SBD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sbd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Scytonema_sp._pcc_10023 Scytonema sp. pcc 10023]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SBD FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbd OCA], [http://pdbe.org/6sbd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sbd RCSB], [http://www.ebi.ac.uk/pdbsum/6sbd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbd ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=L4B:(4~{a}~{R},4~{b}~{S},6~{a}~{R},11~{a}~{R},11~{b}~{S},13~{a}~{R})-1,1,4~{a},6~{a},11~{b}-pentamethyl-9,10-bis(oxidanyl)-+3,4,4~{b},5,6,11,11~{a},12,13,13~{a}-decahydro-2~{H}-indeno[2,1-a]phenanthrene-7-carboxylic+acid'>L4B</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mstE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1680591 Scytonema sp. PCC 10023])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbd OCA], [http://pdbe.org/6sbd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sbd RCSB], [http://www.ebi.ac.uk/pdbsum/6sbd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Class II terpene cyclases, such as oxidosqualene and squalene-hopene cyclases, catalyse some of the most complex polycyclization reactions. They minimally exhibit a beta,gamma-didomain architecture that has been evolutionarily repurposed in a wide range of terpene-processing enzymes and likely resulted from a fusion of unidentified monodomain proteins. Although single domain class I terpene cyclases have already been identified, the corresponding class II counterparts have not been previously reported. Here we present high-resolution X-ray structures of a monodomain class II cyclase, merosterolic acid synthase (MstE). With a minimalistic beta-domain architecture, this cyanobacterial enzyme is able to construct four rings in cytotoxic meroterpenoids with a sterol-like topology. The structures with bound substrate, product, and inhibitor provide detailed snapshots of a cyclization mechanism largely governed by residues located in a noncanonical enzyme region. Our results complement the few known class II cyclase crystal structures, while also indicating that archaic monodomain cyclases might have already catalyzed complex reaction cascades. | ||
| + | |||
| + | A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds.,Moosmann P, Ecker F, Leopold-Messer S, Cahn JKB, Dieterich CL, Groll M, Piel J Nat Chem. 2020 Aug 10. pii: 10.1038/s41557-020-0515-3. doi:, 10.1038/s41557-020-0515-3. PMID:32778689<ref>PMID:32778689</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6sbd" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cahn | + | [[Category: Scytonema sp. pcc 10023]] |
| - | [[Category: Ecker F]] | + | [[Category: Cahn, J K.B]] |
| - | [[Category: Groll M]] | + | [[Category: Ecker, F]] |
| - | [[Category: Leopold-Messer S]] | + | [[Category: Groll, M]] |
| - | [[Category: Moosmann P]] | + | [[Category: Leopold-Messer, S]] |
| - | [[Category: Piel J]] | + | [[Category: Moosmann, P]] |
| + | [[Category: Piel, J]] | ||
| + | [[Category: Alpha6-alpha6 barrel]] | ||
| + | [[Category: Biosynthetic protein]] | ||
| + | [[Category: Marine drug]] | ||
| + | [[Category: Merosterol]] | ||
| + | [[Category: Type ii terpene cyclase]] | ||
Revision as of 06:12, 14 October 2020
Structure of type II terpene cyclase MstE_D109A from Scytonema in complex with merosterolic acid A (product)
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