1qk6

From Proteopedia

(Difference between revisions)

Revision as of 08:52, 21 October 2020

Solution structure of huwentoxin-I by NMR

Structural highlights

1qk6 is a 1 chain structure with sequence from Selenocosmia huwena. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TXH1_HAPSC] Lethal neurotoxin that blocks neuromuscular transmission by binding to the nicotinic acetylcholine receptor. Possess presynaptic activity that affects the release of neurotransmitters from autonomic nerve endings of both cholinergic and adrenergic neuroeffector junctions. Selectively inhibits N-type calcium channel (Cav2.2/CACNA1B) and has only very weak effect on L-type calcium channel (Cav1/CACNA1).^[1] ^[2]

Publication Abstract from PubMed

The three-dimensional structure in aqueous solution of native huwentoxin-I, a neurotoxin from the venom of the spider Selenocosmia huwena, has been determined from two-dimensional H NMR data recorded at 500 and 600 MHz. Structural constraints consisting of interproton distances inferred from NOEs and dihedral angles from spin-spin coupling constants were used as input for distance geometry calculation with the program XPLOR 3.1. The best 10 structures have NOE violations < 0.3 A, dihedral violations < 2 degrees, and pairwise root-mean-square differences of 1.08 (+/- 0.20) A over backbone atoms (N, C alpha, C). The molecule adopts a compact structure consisting of a small triple-stranded antiparallel beta-sheet and five beta-turns. A small hydrophobic patch consisting of Phe 6, Trp 28, and Trp 31 is located on one side of the molecule. All six lysine residues are distributed on the molecular surface. The three disulfide bridges are buried within the molecule. The structure contains an "inhibitor cystine knot motif" which is adopted by several other small proteins, such as omega-conotoxin, agatoxin IVA, and gurmarin.

Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spider Selenocosmia huwena: 2. Three-dimensional structure in solution.,Qu Y, Liang S, Ding J, Liu X, Zhang R, Gu X J Protein Chem. 1997 Aug;16(6):565-74. PMID:9263120^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liang SP, Chen XD, Shu Q, Zhang Y, Peng K. The presynaptic activity of huwentoxin-I, a neurotoxin from the venom of the chinese bird spider Selenocosmia huwena. Toxicon. 2000 Sep;38(9):1237-46. PMID:10736477
↑ Peng K, Chen XD, Liang SP. The effect of Huwentoxin-I on Ca(2+) channels in differentiated NG108-15 cells, a patch-clamp study. Toxicon. 2001 Apr;39(4):491-8. PMID:11024489
↑ Qu Y, Liang S, Ding J, Liu X, Zhang R, Gu X. Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spider Selenocosmia huwena: 2. Three-dimensional structure in solution. J Protein Chem. 1997 Aug;16(6):565-74. PMID:9263120

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qk6"

@@ Line 1: / Line 1: @@
 ==Solution structure of huwentoxin-I by NMR==
-<StructureSection load='1qk6' size='340' side='right' caption='[[1qk6]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='1qk6' size='340' side='right'caption='[[1qk6]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1qk6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Selenocosmia_huwena Selenocosmia huwena]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1qk6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Selenocosmia_huwena Selenocosmia huwena]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1QK6 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk6 OCA], [http://pdbe.org/1qk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qk6 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qk6 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1qk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk6 OCA], [http://pdbe.org/1qk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qk6 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qk6 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Selenocosmia huwena]]
 [[Category: Ding, J]]

1qk6

From Proteopedia

Revision as of 08:52, 21 October 2020

Solution structure of huwentoxin-I by NMR

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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