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3nd2

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Revision as of 09:03, 21 October 2020

Structure of Yeast Importin-beta (Kap95p)

Structural highlights

3nd2 is a 1 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IMB1_YEAST] Required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Serves a receptor for nuclear localization signals. Mediates the nuclear import of histones H2A and H2B.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structure of solenoid proteins facilitates a higher degree of flexibility than most folded proteins. In importin-beta, a nuclear import factor built from 19 tandem HEAT repeats, flexibility plays a crucial role in allowing interactions with a range of different partners. We present a comprehensive analysis of importin-beta flexibility based on a number of different approaches. We determined the crystal structure of unliganded Saccharomyces cerevisiae importin-beta (Kap95) to allow a quantitative comparison with importin-beta bound to different partners. Complementary mutagenesis, small angle X-ray scattering and molecular dynamics studies suggest that the protein samples several conformations in solution. The analyses suggest the flexibility of the solenoid is generated by cumulative small movements along its length. Importin-beta illustrates how solenoid proteins can orchestrate protein interactions in many cellular pathways.

Quantitative structural analysis of importin-beta flexibility: paradigm for solenoid protein structures.,Forwood JK, Lange A, Zachariae U, Marfori M, Preast C, Grubmuller H, Stewart M, Corbett AH, Kobe B Structure. 2010 Sep 8;18(9):1171-83. PMID:20826343^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mosammaparast N, Jackson KR, Guo Y, Brame CJ, Shabanowitz J, Hunt DF, Pemberton LF. Nuclear import of histone H2A and H2B is mediated by a network of karyopherins. J Cell Biol. 2001 Apr 16;153(2):251-62. PMID:11309407
↑ Forwood JK, Lange A, Zachariae U, Marfori M, Preast C, Grubmuller H, Stewart M, Corbett AH, Kobe B. Quantitative structural analysis of importin-beta flexibility: paradigm for solenoid protein structures. Structure. 2010 Sep 8;18(9):1171-83. PMID:20826343 doi:10.1016/j.str.2010.06.015

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nd2"

@@ Line 1: / Line 1: @@
 ==Structure of Yeast Importin-beta (Kap95p)==
-<StructureSection load='3nd2' size='340' side='right' caption='[[3nd2]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+<StructureSection load='3nd2' size='340' side='right'caption='[[3nd2]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nd2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ND2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ND2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nd2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ND2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3ND2 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nd2 OCA], [http://pdbe.org/3nd2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nd2 RCSB], [http://www.ebi.ac.uk/pdbsum/3nd2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nd2 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3nd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nd2 OCA], [http://pdbe.org/3nd2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nd2 RCSB], [http://www.ebi.ac.uk/pdbsum/3nd2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nd2 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 29: / Line 29: @@
 ==See Also==
-*[[Importin|Importin]]
+*[[Importin 3D structures|Importin 3D structures]]
 == References ==
 <references/>
@@ Line 35: / Line 35: @@
 </StructureSection>
 [[Category: Atcc 18824]]
+[[Category: Large Structures]]
 [[Category: Forwood, J K]]
 [[Category: Kobe, B]]

3nd2

From Proteopedia

Revision as of 09:03, 21 October 2020

Structure of Yeast Importin-beta (Kap95p)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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