1cwe

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1cwe.jpg|left|200px]]
[[Image:1cwe.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1cwe |SIZE=350|CAPTION= <scene name='initialview01'>1cwe</scene>, resolution 2.3&Aring;
+
The line below this paragraph, containing "STRUCTURE_1cwe", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=PM3:2-AMINO-3-(4-PHOSPHONOMETHYL-PHENYL)-PROPIONIC+ACID'>PM3</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1cwe| PDB=1cwe | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cwe OCA], [http://www.ebi.ac.uk/pdbsum/1cwe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cwe RCSB]</span>
+
-
}}
+
'''HUMAN P56LCK TYROSINE KINASE COMPLEXED WITH PHOSPHONOPEPTIDE'''
'''HUMAN P56LCK TYROSINE KINASE COMPLEXED WITH PHOSPHONOPEPTIDE'''
Line 27: Line 24:
[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Mikol, V.]]
[[Category: Mikol, V.]]
-
[[Category: complex (phosphotransferase/peptide)]]
+
[[Category: Phosphotransferase]]
-
[[Category: phosphotransferase]]
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:11:08 2008''
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:29:49 2008''
+

Revision as of 10:11, 2 May 2008

Image:1cwe.jpg

Template:STRUCTURE 1cwe

HUMAN P56LCK TYROSINE KINASE COMPLEXED WITH PHOSPHONOPEPTIDE


Overview

Src homology-2 (SH2) domains are protein modules found within a wide variety of cytoplasmic signalling molecules that bind with high affinity to phosphotyrosyl-containing protein sequences. In order to develop SH2 inhibitors that contain phosphotyrosyl analogues resistant to cellular phosphatases, we have solved the crystal structures of the SH2 domain of p56lck in separate complexes with two high-affinity p-(phosphonomethyl)phenylalanine-containing peptides. The structures have been determined at 2.3 A and 2.25 A, and refined to crystallographic R-factors of 19.2% and 18.5%, respectively. The conformation of the SH2 domain of p56lck is essentially similar to that observed in Src and Lck complexed with a phosphotyrosine-containing peptide except in some loops and especially in the loop that connects the second and third beta-strands. This loop, which was involved in hydrogen-bond interactions with the phosphotyrosine moiety, has moved away in the phosphonopeptide complexes as a rigid body by about 7 A on two hinges leaving the tyrosine phosphate mimetic moiety accessible to the solvent. Some intramolecular hydrogen bonds with other residues of the third and fourth beta-strands stabilize an open conformation of the lid, suggesting a flap mechanism for peptide binding.

About this Structure

1CWE is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The crystal structures of the SH2 domain of p56lck complexed with two phosphonopeptides suggest a gated peptide binding site., Mikol V, Baumann G, Keller TH, Manning U, Zurini MG, J Mol Biol. 1995 Feb 17;246(2):344-55. PMID:7532720 Page seeded by OCA on Fri May 2 13:11:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cwe"
Personal tools