1cx6

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[[Image:1cx6.gif|left|200px]]
[[Image:1cx6.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1cx6 |SIZE=350|CAPTION= <scene name='initialview01'>1cx6</scene>, resolution 2.01&Aring;
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The line below this paragraph, containing "STRUCTURE_1cx6", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= GENE E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])
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-->
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|DOMAIN=
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{{STRUCTURE_1cx6| PDB=1cx6 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cx6 OCA], [http://www.ebi.ac.uk/pdbsum/1cx6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cx6 RCSB]</span>
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}}
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'''T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE'''
'''T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE'''
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[[Category: Gassner, N C.]]
[[Category: Gassner, N C.]]
[[Category: Matthews, B W.]]
[[Category: Matthews, B W.]]
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[[Category: hydrolase (o-glycosyl)]]
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[[Category: Protein engineering]]
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[[Category: protein engineering]]
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[[Category: Protein folding]]
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[[Category: protein folding]]
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[[Category: Selenomethionine core mutant]]
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[[Category: selenomethionine core mutant]]
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[[Category: T4 lysozyme]]
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[[Category: t4 lysozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:12:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:30:19 2008''
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Revision as of 10:12, 2 May 2008

Image:1cx6.gif

Template:STRUCTURE 1cx6

T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE


Overview

The availability of a series of phage T4 lysozymes with up to 14 methionine residues incorporated within the protein has made it possible to systematically compare the effect on protein stability of selenomethionine relative to methionine. Wild-type lysozyme contains two fully buried methionine residues plus three more on the surface. The substitution of these methionine residues with selenomethionine slightly stabilizes the protein. As more and more methionine residues are substituted into the protein, there is a progressive loss of stability. This is, however, increasingly offset in the selenomethionine variants, ultimately resulting in a differential increase in melting temperature of about 7 degrees C. This increase, corresponding to about 0.25 kcal/mol per substitution, is in reasonable agreement with the difference in the solvent transfer free energy between the two amino acids.

About this Structure

1CX6 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Substitution with selenomethionine can enhance the stability of methionine-rich proteins., Gassner NC, Baase WA, Hausrath AC, Matthews BW, J Mol Biol. 1999 Nov 19;294(1):17-20. PMID:10556025 Page seeded by OCA on Fri May 2 13:12:39 2008

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