1cx7

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[[Image:1cx7.gif|left|200px]]
[[Image:1cx7.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1cx7 |SIZE=350|CAPTION= <scene name='initialview01'>1cx7</scene>, resolution 1.94&Aring;
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The line below this paragraph, containing "STRUCTURE_1cx7", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= GENE E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])
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-->
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|DOMAIN=
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{{STRUCTURE_1cx7| PDB=1cx7 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cx7 OCA], [http://www.ebi.ac.uk/pdbsum/1cx7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cx7 RCSB]</span>
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}}
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'''T4 LYSOZYME METHIONINE CORE MUTANT'''
'''T4 LYSOZYME METHIONINE CORE MUTANT'''
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[[Category: Lu, J.]]
[[Category: Lu, J.]]
[[Category: Matthews, B W.]]
[[Category: Matthews, B W.]]
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[[Category: hydrolase (o-glycosyl)]]
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[[Category: Methionine core mutant]]
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[[Category: methionine core mutant]]
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[[Category: Protein engineering]]
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[[Category: protein engineering]]
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[[Category: Protein folding]]
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[[Category: protein folding]]
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[[Category: T4 lysozyme]]
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[[Category: t4 lysozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:12:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:30:19 2008''
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Revision as of 10:12, 2 May 2008

Image:1cx7.gif

Template:STRUCTURE 1cx7

T4 LYSOZYME METHIONINE CORE MUTANT


Overview

Using heavily methionine-substituted T4 lysozyme as an example, it is shown how the addition or deletion of a small number of methionines can simplify the location of selenium sites for use in MAD phasing. By comparing the X-ray data for a large number of singly substituted lysozymes, it is shown that the optimal amino acid to be substituted by methionine is leucine, followed, in order of preference, by phenylalanine, isoleucine and valine. The identification of leucine as the first choice agrees with the ranking suggested by the Dayhoff mutation probability, i.e. by the frequency of amino-acid substitutions in the sequences of related proteins. The ranking of the second and subsequent choices, however, differ significantly.

About this Structure

1CX7 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Use of differentially substituted selenomethionine proteins in X-ray structure determination., Gassner NC, Matthews BW, Acta Crystallogr D Biol Crystallogr. 1999 Dec;55(Pt 12):1967-70. PMID:10666571 Page seeded by OCA on Fri May 2 13:12:44 2008

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