5z1g

From Proteopedia

(Difference between revisions)

Revision as of 21:13, 28 October 2020

Structure of the Brx1 and Ebp2 complex

Structural highlights

5z1g is a 4 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	EBP2, YKL172W, YKL636 (Baker's yeast), BRX1, YOL077C (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[EBP2_YEAST] Required for the processing of the 27S pre-rRNA. Probably involved in the step of the processing of the 27 SA precursor into the 27 SB intermediate.^[1] ^[2] [BRX1_YEAST] Required for biogenesis of the 60S ribosomal subunit.^[3]

Publication Abstract from PubMed

Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 A resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits.

Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.,Zhou D, Zhu X, Zheng S, Tan D, Dong MQ, Ye K Protein Cell. 2018 Mar 19. pii: 10.1007/s13238-018-0526-7. doi:, 10.1007/s13238-018-0526-7. PMID:29557065^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Huber MD, Dworet JH, Shire K, Frappier L, McAlear MA. The budding yeast homolog of the human EBNA1-binding protein 2 (Ebp2p) is an essential nucleolar protein required for pre-rRNA processing. J Biol Chem. 2000 Sep 15;275(37):28764-73. doi: 10.1074/jbc.M000594200. PMID:10849420 doi:http://dx.doi.org/10.1074/jbc.M000594200
↑ Tsujii R, Miyoshi K, Tsuno A, Matsui Y, Toh-e A, Miyakawa T, Mizuta K. Ebp2p, yeast homologue of a human protein that interacts with Epstein-Barr virus nuclear antigen 1, is required for pre-rRNA processing and ribosomal subunit assembly. Genes Cells. 2000 Jul;5(7):543-53. PMID:10947841
↑ Kaser A, Bogengruber E, Hallegger M, Doppler E, Lepperdinger G, Jantsch M, Breitenbach M, Kreil G. Brix from xenopus laevis and brx1p from yeast define a new family of proteins involved in the biogenesis of large ribosomal subunits. Biol Chem. 2001 Dec;382(12):1637-47. doi: 10.1515/BC.2001.199. PMID:11843177 doi:http://dx.doi.org/10.1515/BC.2001.199
↑ Zhou D, Zhu X, Zheng S, Tan D, Dong MQ, Ye K. Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate. Protein Cell. 2018 Mar 19. pii: 10.1007/s13238-018-0526-7. doi:, 10.1007/s13238-018-0526-7. PMID:29557065 doi:http://dx.doi.org/10.1007/s13238-018-0526-7

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5z1g"

@@ Line 1: / Line 1: @@
 ==Structure of the Brx1 and Ebp2 complex==
-<StructureSection load='5z1g' size='340' side='right' caption='[[5z1g]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
+<StructureSection load='5z1g' size='340' side='right'caption='[[5z1g]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5z1g]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z1G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z1G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5z1g]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z1G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z1G FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z1g OCA], [http://pdbe.org/5z1g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z1g RCSB], [http://www.ebi.ac.uk/pdbsum/5z1g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z1g ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EBP2, YKL172W, YKL636 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), BRX1, YOL077C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z1g OCA], [http://pdbe.org/5z1g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z1g RCSB], [http://www.ebi.ac.uk/pdbsum/5z1g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z1g ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5z1g" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Ribosome biogenesis protein|Ribosome biogenesis protein]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
+[[Category: Large Structures]]
 [[Category: Ye, K]]
 [[Category: Zheng, S]]

5z1g

From Proteopedia

Revision as of 21:13, 28 October 2020

Structure of the Brx1 and Ebp2 complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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