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5z68

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Structure of the recombination mediator protein RecF-ATP in RecFOR pathway

Structural highlights

5z68 is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RECF_CALS4] The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.

Publication Abstract from PubMed

RecF is a principal member of the RecF pathway. It interacts with RecO and RecR to initiate homologous recombination by loading RecA recombinases on single-stranded DNA and displacing single-stranded DNA-binding proteins. As an ATP-binding cassette ATPase, RecF exhibits ATP-dependent dimerization and structural homology with Rad50 and SMC proteins. However, the mechanism and action pattern of RecF ATP-dependent dimerization remains unclear. Here, We determined three crystal structures of TTERecF, TTERecF-ATP and TTERecF-ATPS from Thermoanaerobacter tengcongensis that reveal a novel ATP-driven RecF dimerization. RecF contains a positively charged tunnel on its dimer interface that is essential to ATP binding. Our structural and biochemical data indicate that the Walker A motif serves as a switch and plays a key role in ATP binding and RecF dimerization. Furthermore, Biolayer interferometry assay results showed that the TTERecF interacted with ATP and formed a dimer, displaying a higher affinity for DNA than that of the TTERecF monomer. Overall, our results provide a solid structural basis for understanding the process of RecF binding with ATP and the functional mechanism of ATP-dependent RecF dimerization.

ATP-dependent conformational change in ABC-ATPase RecF serves as a switch in DNA repair.,Tang Q, Liu YP, Shan HH, Tian LF, Zhang JZ, Yan XX Sci Rep. 2018 Feb 1;8(1):2127. doi: 10.1038/s41598-018-20557-0. PMID:29391496^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tang Q, Liu YP, Shan HH, Tian LF, Zhang JZ, Yan XX. ATP-dependent conformational change in ABC-ATPase RecF serves as a switch in DNA repair. Sci Rep. 2018 Feb 1;8(1):2127. doi: 10.1038/s41598-018-20557-0. PMID:29391496 doi:http://dx.doi.org/10.1038/s41598-018-20557-0

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5z68"

@@ Line 1: / Line 1: @@
 ==Structure of the recombination mediator protein RecF-ATP in RecFOR pathway==
-<StructureSection load='5z68' size='340' side='right' caption='[[5z68]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+<StructureSection load='5z68' size='340' side='right'caption='[[5z68]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5z68]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z68 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5z68]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z68 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z68 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z68 OCA], [http://pdbe.org/5z68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z68 RCSB], [http://www.ebi.ac.uk/pdbsum/5z68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z68 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z68 OCA], [http://pdbe.org/5z68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z68 RCSB], [http://www.ebi.ac.uk/pdbsum/5z68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z68 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Liu, Y P]]
 [[Category: Tang, Q]]

5z68

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Current revision

Structure of the recombination mediator protein RecF-ATP in RecFOR pathway

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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