6a67

From Proteopedia

(Difference between revisions)

Revision as of 21:26, 28 October 2020

Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody FLD21.140

Structural highlights

6a67 is a 6 chain structure with sequence from Human and Influenza a virus (a/thailand/1(kan-1)/2004(h5n1)). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q6Q794_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[SAAS:SAAS01039674] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324]

Publication Abstract from PubMed

The highly pathogenic avian influenza virus H5N1 is a major threat to global public health and therefore a high-priority target of current vaccine development. The receptor-binding site (RBS) on the globular head of hemagglutinin (HA) in the viral envelope is one of the major target sites for antibody recognition against H5N1 and other influenza viruses. Here, we report the identification and characterization of a pair of human RBS-specific antibodies, designated FLD21.140 and AVFluIgG03, that are mutually complementary in their neutralizing activities against a diverse panel of H5N1 viruses. Crystallographic analysis and site-directed mutagenesis revealed that the two antibodies share a similar RBS-binding mode and their individual specificities are governed by residues at positions 133a, 144, and 145. Specifically, FLD21.140 preferred Leu-133a/Lys-144/Ser-145, whereas AVFluIgG03 favored Ser-133a /Thr-144/Pro-145 residue triplets, both of which perfectly matched the most prevalent residues in viruses from epidemic-originating regions. Of note, according to an analysis of 3758 H5 HAs sequences available in the Influenza Virus Database at the National Center for Biotechnology Information, the residues Leu-133a/Ser-133a and Ser-145/Pro-145 constituted more than 87.6% and 99.3% of all residues at these two positions, respectively. Taken together, our results provide a structural understanding for the neutralizing complementarity of these two antibodies and improve our understanding of the RBS-specific antibody response against H5N1 infection in humans.

Complementary recognition of the receptor binding site of highly pathogenic H5N1 influenza viruses by two human neutralizing antibodies.,Zuo Y, Wang P, Sun J, Guo S, Wang G, Zuo T, Fan S, Zhou P, Liang M, Shi X, Wang X, Zhang L J Biol Chem. 2018 Aug 28. pii: RA118.004604. doi: 10.1074/jbc.RA118.004604. PMID:30154240^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zuo Y, Wang P, Sun J, Guo S, Wang G, Zuo T, Fan S, Zhou P, Liang M, Shi X, Wang X, Zhang L. Complementary recognition of the receptor binding site of highly pathogenic H5N1 influenza viruses by two human neutralizing antibodies. J Biol Chem. 2018 Aug 28. pii: RA118.004604. doi: 10.1074/jbc.RA118.004604. PMID:30154240 doi:http://dx.doi.org/10.1074/jbc.RA118.004604

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6a67"

@@ Line 1: / Line 1: @@
 ==Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody FLD21.140==
-<StructureSection load='6a67' size='340' side='right' caption='[[6a67]], [[Resolution|resolution]] 2.33&Aring;' scene=''>
+<StructureSection load='6a67' size='340' side='right'caption='[[6a67]], [[Resolution|resolution]] 2.33&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6a67]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/thailand/1(kan-1)/2004(h5n1)) Influenza a virus (a/thailand/1(kan-1)/2004(h5n1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A67 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6a67]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/thailand/1(kan-1)/2004(h5n1)) Influenza a virus (a/thailand/1(kan-1)/2004(h5n1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A67 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6A67 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a67 OCA], [http://pdbe.org/6a67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a67 RCSB], [http://www.ebi.ac.uk/pdbsum/6a67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a67 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a67 OCA], [http://pdbe.org/6a67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a67 RCSB], [http://www.ebi.ac.uk/pdbsum/6a67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a67 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 6a67" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]]
 == References ==
 <references/>
@@ Line 23: / Line 26: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Sun, J]]
 [[Category: Wang, P]]

6a67

From Proteopedia

Revision as of 21:26, 28 October 2020

Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody FLD21.140

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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