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1e9t

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Revision as of 07:49, 4 November 2020

High resolution solution structure of human intestinal trefoil factor

Structural highlights

1e9t is a 1 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TFF3_HUMAN] Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The secreted proteins intestinal trefoil factor (ITF, 59 residues), pS2 (60 residues), and spasmolytic polypeptide (SP, 106 residues) form a small family of trefoil domain-containing mammalian cell motility factors, which are essential for the maintenance of all mucous-coated epithelial surfaces. We have used 1H NMR spectroscopy to determine the high-resolution structure of human ITF, which has allowed detailed structural comparisons with the other trefoil cell motility factors. The conformation of residues 10-53 of hITF is determined to high precision, but the structure of the N- and C-terrminal residues is poorly defined by the NMR data, which is probably indicative of significant mobility. The core of the trefoil domain in hITF consists of a two-stranded antiparallel beta-sheet (Cys 36 to Asp 39 and Trp 47 to Lys 50), which is capped by an irregular loop and forms a central hairpin (loop 3). The beta-sheet is preceded by a short alpha-helix (Lys 29 to Arg 34), with the majority of the remainder of the domain contained in two loops formed from His 25 to Pro 28 (loop 2) and Ala 12 to Arg 18 (loop 1), which lie on either side of the central hairpin. The region formed by the surface of loop 2, the cleft between loop 2 and loop 3, and the adjacent face of loop 3 has previously been proposed to form the functional site of trefoil domains. Detailed comparisons of the backbone conformations and surface features of the family of trefoil cell motility factors (porcine SP, pS2, and hITF) have identified significant structural and electrostatic differences in the loop 2/loop 3 regions, which suggest that each trefoil protein has a specific target or group of target molecules.

High-resolution solution structure of human intestinal trefoil factor and functional insights from detailed structural comparisons with the other members of the trefoil family of mammalian cell motility factors.,Lemercinier X, Muskett FW, Cheeseman B, McIntosh PB, Thim L, Carr MD Biochemistry. 2001 Aug 14;40(32):9552-9. PMID:11583154^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Oertel M, Graness A, Thim L, Buhling F, Kalbacher H, Hoffmann W. Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor. Am J Respir Cell Mol Biol. 2001 Oct;25(4):418-24. PMID:11694446 doi:http://dx.doi.org/10.1165/ajrcmb.25.4.4429
↑ Lemercinier X, Muskett FW, Cheeseman B, McIntosh PB, Thim L, Carr MD. High-resolution solution structure of human intestinal trefoil factor and functional insights from detailed structural comparisons with the other members of the trefoil family of mammalian cell motility factors. Biochemistry. 2001 Aug 14;40(32):9552-9. PMID:11583154

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1e9t"

@@ Line 1: / Line 1: @@
 ==High resolution solution structure of human intestinal trefoil factor==
-<StructureSection load='1e9t' size='340' side='right' caption='[[1e9t]], [[NMR_Ensembles_of_Models | 85 NMR models]]' scene=''>
+<StructureSection load='1e9t' size='340' side='right'caption='[[1e9t]], [[NMR_Ensembles_of_Models | 85 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1e9t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E9T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E9T FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1e9t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E9T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1E9T FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e9t OCA], [http://pdbe.org/1e9t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e9t RCSB], [http://www.ebi.ac.uk/pdbsum/1e9t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e9t ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1e9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e9t OCA], [http://pdbe.org/1e9t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e9t RCSB], [http://www.ebi.ac.uk/pdbsum/1e9t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e9t ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 32: / Line 32: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Carr, M]]
 [[Category: Cheeseman, B]]

1e9t

From Proteopedia

Revision as of 07:49, 4 November 2020

High resolution solution structure of human intestinal trefoil factor

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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