3o0r

From Proteopedia

(Difference between revisions)

Revision as of 08:09, 4 November 2020

Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment

Structural highlights

3o0r is a 4 chain structure with sequence from Mus musculus and Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Activity:	Nitric-oxide reductase (cytochrome c), with EC number 1.7.2.5
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NORC_PSEAE] Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). [NORB_PSEAE] Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen reduction is probably coupled to electron transport phosphorylation (By similarity).

Publication Abstract from PubMed

Nitric oxide reductase (NOR) is an iron-containing enzyme that catalyzes the reduction of nitric oxide (NO) to generate a major greenhouse gas, nitrous oxide (N(2)O). Here, we report the crystal structure of NOR from Pseudomonas aeruginosa at 2.7 angstrom resolution. The structure reveals details of the catalytic binuclear center. The non-heme iron (Fe(B)) is coordinated by three His and one Glu ligands, but a His-Tyr covalent linkage common in cytochrome oxidases (COX) is absent. This structural characteristic is crucial for NOR reaction. Although the overall structure of NOR is closely related to COX, neither the D- nor K-proton pathway, which connect the COX active center to the intracellular space, was observed. Protons required for the NOR reaction are probably provided from the extracellular side.

Structural basis of biological N2O generation by bacterial nitric oxide reductase.,Hino T, Matsumoto Y, Nagano S, Sugimoto H, Fukumori Y, Murata T, Iwata S, Shiro Y Science. 2010 Dec 17;330(6011):1666-70. Epub 2010 Nov 25. PMID:21109633^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hino T, Matsumoto Y, Nagano S, Sugimoto H, Fukumori Y, Murata T, Iwata S, Shiro Y. Structural basis of biological N2O generation by bacterial nitric oxide reductase. Science. 2010 Dec 17;330(6011):1666-70. Epub 2010 Nov 25. PMID:21109633 doi:10.1126/science.1195591

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3o0r"

@@ Line 1: / Line 1: @@
 ==Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment==
-<StructureSection load='3o0r' size='340' side='right' caption='[[3o0r]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='3o0r' size='340' side='right'caption='[[3o0r]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3o0r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O0R FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3o0r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0R OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3O0R FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_reductase_(cytochrome_c) Nitric-oxide reductase (cytochrome c)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.5 1.7.2.5] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o0r OCA], [http://pdbe.org/3o0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o0r RCSB], [http://www.ebi.ac.uk/pdbsum/3o0r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o0r ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3o0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o0r OCA], [http://pdbe.org/3o0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o0r RCSB], [http://www.ebi.ac.uk/pdbsum/3o0r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o0r ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 ==See Also==
 *[[Antibody 3D structures|Antibody 3D structures]]
-*[[Nitrite reductase|Nitrite reductase]]
+*[[Nitrite reductase 3D structures|Nitrite reductase 3D structures]]
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
 [[Category: Pseudomonas aeruginosa]]

3o0r

From Proteopedia

Revision as of 08:09, 4 November 2020

Crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in complex with antibody fragment

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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