7k1w
From Proteopedia
(Difference between revisions)
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==PIKfyve/Fig4/Vac14 complex centered on Fig4 - map3== | ==PIKfyve/Fig4/Vac14 complex centered on Fig4 - map3== | ||
| - | <StructureSection load='7k1w' size='340' side='right'caption='[[7k1w]]' scene=''> | + | <StructureSection load='7k1w' size='340' side='right'caption='[[7k1w]], [[Resolution|resolution]] 5.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K1W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7K1W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7k1w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K1W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7K1W FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7k1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k1w OCA], [http://pdbe.org/7k1w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7k1w RCSB], [http://www.ebi.ac.uk/pdbsum/7k1w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7k1w ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7k1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k1w OCA], [http://pdbe.org/7k1w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7k1w RCSB], [http://www.ebi.ac.uk/pdbsum/7k1w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7k1w ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The phosphoinositide PI(3,5)P2, generated exclusively by the PIKfyve lipid kinase complex, is key for lysosomal biology. Here, we explore how PI(3,5)P2 levels within cells are regulated. We find the PIKfyve complex comprises five copies of the scaffolding protein Vac14 and one copy each of the lipid kinase PIKfyve, generating PI(3,5)P2 from PI3P and the lipid phosphatase Fig4, reversing the reaction. Fig4 is active as a lipid phosphatase in the ternary complex, whereas PIKfyve within the complex cannot access membrane-incorporated phosphoinositides due to steric constraints. We find further that the phosphoinositide-directed activities of both PIKfyve and Fig4 are regulated by protein-directed activities within the complex. PIKfyve autophosphorylation represses its lipid kinase activity and stimulates Fig4 lipid phosphatase activity. Further, Fig4 is also a protein phosphatase acting on PIKfyve to stimulate its lipid kinase activity, explaining why catalytically active Fig4 is required for maximal PI(3,5)P2 production by PIKfyve in vivo. | ||
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| + | Insights into Lysosomal PI(3,5)P2 Homeostasis from a Structural-Biochemical Analysis of the PIKfyve Lipid Kinase Complex.,Lees JA, Li P, Kumar N, Weisman LS, Reinisch KM Mol Cell. 2020 Oct 15. pii: S1097-2765(20)30686-9. doi:, 10.1016/j.molcel.2020.10.003. PMID:33098764<ref>PMID:33098764</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7k1w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Lees | + | [[Category: Lees, J A]] |
| - | [[Category: Li P]] | + | [[Category: Li, P]] |
| - | [[Category: Reinisch | + | [[Category: Reinisch, K M]] |
| + | [[Category: Lipid binding protein]] | ||
| + | [[Category: Lipid kinase]] | ||
| + | [[Category: Lipid phosphatase]] | ||
| + | [[Category: Protein complex]] | ||
Revision as of 08:19, 11 November 2020
PIKfyve/Fig4/Vac14 complex centered on Fig4 - map3
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