N-acetylglucosamine-1-phosphate uridyltransferase

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== Function ==
== Function ==
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'''N-acetylglucosamine-1-phosphate uridyltransferase''' (GlmU} or '''bifunctional protein GlmU''' is a bifunctional acetyltransferase/uridyltransferase that catalyzes the formation of UDP-GlcNAc from glucosamine-1-phosphate (G1P)(<ref>PMID:19237750</ref>.)
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'''N-acetylglucosamine-1-phosphate uridyltransferase''' (GlmU) or '''bifunctional protein GlmU''' is a bifunctional acetyltransferase/uridyltransferase that catalyzes the formation of UDP-GlcNAc from glucosamine-1-phosphate (G1P)(<ref>PMID:19237750</ref>.)
== Relevance ==
== Relevance ==
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== Structural highlights ==
== Structural highlights ==
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The UDP-GlcNAc binds to GlmU in the enzyme's N-terminal domain and the majority of the key active site residues are highly conserved across the bacterial GlmU family. The 3D structure of the complex shows an exposed <scene name='84/841078/Cv/3'>uracil binding pocket</scene>, GlcNAc interacting pocket and a lipophilic pocket <ref>PMID:18029420</ref>.
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The UDP-GlcNAc binds to GlmU in the enzyme's N-terminal domain and the majority of the key active site residues are highly conserved across the bacterial GlmU family. The 3D structure of the complex shows an exposed <scene name='84/841078/Cv/4'>uracil binding pocket</scene>, GlcNAc interacting pocket and a lipophilic pocket <ref>PMID:18029420</ref>.
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Revision as of 15:55, 11 November 2020

GlmU complex with its reaction product UDP-GlcNAC, PEG, Mg++, So4, tetraethylene glycol and triethylene glycol (PDB code 2v0j)

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3D structures of GlmU

Updated on 11-November-2020

References

  1. Zhang Z, Bulloch EM, Bunker RD, Baker EN, Squire CJ. Structure and function of GlmU from Mycobacterium tuberculosis. Acta Crystallogr D Biol Crystallogr. 2009 Mar;65(Pt 3):275-83. Epub 2009, Feb 20. PMID:19237750 doi:10.1107/S0907444909001036
  2. . PMID:318573680
  3. Mochalkin I, Lightle S, Zhu Y, Ohren JF, Spessard C, Chirgadze NY, Banotai C, Melnick M, McDowell L. Characterization of substrate binding and catalysis in the potential antibacterial target N-acetylglucosamine-1-phosphate uridyltransferase (GlmU). Protein Sci. 2007 Dec;16(12):2657-66. PMID:18029420 doi:http://dx.doi.org/16/12/2657

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Alexander Berchansky, Michal Harel

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